Recombinant Protein Purification Products & Technology
Avantor®’s J.T.Baker® brand offers the only cGMP manufactured IPTG to ICHQ7 standards. That provides biopharma researchers and manufacturers with a solution to help scale recombinant vaccines from research and development to clinical and full-scale manufacturing with speed, quality, and confidence.Buy now
Recombinant proteins workflow
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What Are Recombinant Proteins?
Recombinant proteins are artificially made in a laboratory using recombinant DNA technology. Cells are typically transfected with a DNA vector, cultured to transcribe and translate the protein, lysed, and purified. There are various ways to complete recombinant protein purification to maximize protein yield.
Advances in recombinant protein technology and research have expanded the applications of recombinant proteins to include their use as diagnostic reagents, therapeutics, and vaccines.
How Do Proteins Become Recombinant Proteins?
Proteins are the result of the expression of DNA. Recombinant proteins are the result of the expression of recombinant DNA (rDNA) within living cells. Laboratory methods used to construct recombinant DNA combine at least two DNA fragments from different sources. The recombinant DNA is then inserted into a vector and allowed to replicate inside a living cell.
Recombinant Protein Production
Recombinant proteins are manufactured using microbial (E. coli) expression systems. They represent a group of smaller protein molecules that include cytokine (erythropoietin or EPO), growth factors (insulin), and enzymes (Urokinase), among others.
Target proteins are secreted intracellularly using E. coli expression systems and may be challenging to recover. These insoluble proteins, along with other contaminants, are in inclusion bodies within the bacterial cytoplasm. Lysing the bacteria causes them to release the insoluble inclusion bodies.
After the proteins are solubilized and isolated, other mechanical and chemical additions allow proper protein folding. That results in biologically active proteins. Recombinant protein purification utilizing chromatography steps is comparable to that used for other biologics.
Find Everything You Need for Recombinant Protein Production at Avantor
Avantor® offers a broad range of products to aid in the recombinant protein process, including fermentation products to support cell growth and excipients, such as polysorbates, in the formulation range of chromatography media to help in recombinant protein purification.