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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   NEB* Golden Gate Assembly kit, Updated to include BsaI-HFv2, Seamless cloning -no scar, plasmid with T7/SP6 promoters, Efficient with regions with high GC content and areas of repeats, Compatible w/ broad range of fragment sizes < 100 bp to > 15 kb, 20 rxns
Catalog Number: 76326-028
Supplier: New England Biolabs (NEB)



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Description:   High efficiency UA PCR cloning, features DNA topoisomerase I and Cre recombinase technologies 20 reactions
Catalog Number: 99901-042
Supplier: Agilent Technologies



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Description:   The kit provides a rapid and reliable method for site directed mutagenesis. 1 kit (30 rxn)
Catalog Number: 99900-044
Supplier: Agilent Technologies



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Catalog Number: ST200517
Supplier: Agilent Technologies



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MSDS SDS    
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Description:   For Academic use only. Novel, robust technology for complex, multi-site mutagenesis in a single reaction. 1 Kit (10 Rxn)
Catalog Number: 99900-024
Supplier: Agilent Technologies



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MSDS SDS    
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Description:   DNA Ligase T4 Molecular Biology Grade, Store vial at -20degree C prior to opening, Application Note: One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37degree C, 100units
Catalog Number: 10801-154
Supplier: Rockland Immunochemical



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Description:   NEB* PCR Cloning Kit(Without Competent Cells), Components: Cloning Mix 1 & 2, Linearized pMiniT* Vector, Amplicon Cloning Control, Cloning Analysis Forward Primer, Cloning Analysis Reverse Primer, Easy cloning of all PCR products, including blunt & TA ends, Size: 20 reactions
Catalog Number: 102969-096
Supplier: New England Biolabs (NEB)


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Description:   The kit provides a rapid and reliable method for site directed mutagenesis. 1 kit (10 rxn)
Catalog Number: 99900-052
Supplier: Agilent Technologies



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Description:   Packaging efficiency: 1e9 pfu/ug. Preferentially packages phage with large inserts; For genomic lambda and cosmid libraries Contains: Gigapack III XL packaging extract, control lambda DNA and control host strain 4 vials
Catalog Number: 99900-312
Supplier: Agilent Technologies



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Description:   For Academic Use Only. Novel, Robust Technology For Complex, Multi-Site Mutagenesis In A Single Reaction. Contains: Dna Polymerase Blend, Template Digestion Enzyme, Competent Cells, Positive Controls. 1 Kit Contains 30 Reactions.
Catalog Number: 99900-566
Supplier: Agilent Technologies



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MSDS SDS    
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Description:   High efficiency blunt-end PCR cloning kit features PfuUltra * II HS DNA Polymerase, DNA topoisomerase I, and Cre recombinase technologies 20 reactions
Catalog Number: 95040-726
Supplier: Agilent Technologies



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Description:   T4 DNA Ligase
Catalog Number: 95040-312
Supplier: Cytiva



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Description:   DNA Ligase T4 Molecular Biology Grade, Store vial at -20degree C prior to opening, Application Note: One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37degree C, 500units
Catalog Number: 10801-156
Supplier: Rockland Immunochemical



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Description:   20 reactions
Catalog Number: 101417-934
Supplier: New England Biolabs (NEB)


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Description:   Gibson Assembly* HiFi 1 Step Cloning Kit, Component: GA HiFi 1 Step Master Mix (2X),250 uL,GA Positive Control (2X), 25 uL (5 Control Reactions),Detection Range: 200 bp to 100 kb, up to 5 fragments in one reaction, Method: One-step, Quantity: 50 reactions
Catalog Number: 10820-614
Supplier: TELESIS BIO



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MSDS SDS    
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Description:   Quick Blunting Kit - 20 rxns
Catalog Number: 101417-942
Supplier: New England Biolabs (NEB)


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