QUANTIFOIL® is a carbon film for electron microscopy or low-energy electron point source microscopy.
- Mounted on copper, nickel or gold grids
- Foil is 12 nm thick
- Thicknesses between 10 and 25 nm upon request
QUANTIFOIL® is a perforated support foil with pre-defined hole size, shape and arrangement that has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) when compared with conventional holey film. QUANTIFOIL® with circular holes is used in cryo-electron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness.
The hole size chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. Assessment of the image quality is easier when foil is included in the picture, because the power spectrum of a foil is stronger than that of unsupported ice.
QUANTIFOIL® R 5/10 has a hole size of 5 µm. Space between holes is 10 µm. Center to center is 15 µm.
QUANTIFOIL® R 5/20 has a hole size of 5 µm. Space between holes is 20 µm. Center to center is 25 µm.
The larger holes (3.5, 5 µm) are suitable at magnification around 20000×.
Ordering information: QUANTIFOIL® is delivered as a carbon foil; it can be reinforced with plastic film. The grids are packed with their coated side toward the center of the grid box.