Synonyms:
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol
0219402995
0219402980
0219402991
0219402990
0219402994
IC0219402990EA
57.93
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IC0219402990
IC0219402980
IC0219402991
IC0219402994
IC0219402995
IPTG (isopropyl-β-D-thiogalactopyranoside) ≥99% for molecular biology
IPTG (Isopropyl-β-D-thiogalactopyranoside)
Isopropyl b-D-1-thiogalactopyranoside is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. It functions by binding to the lac repressor and altering its conformation. This inactivation prevents the repression of the b-galactosidase coding lac gene.
- Storage temperature: -20 °C, desiccate
Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.