Synonyms:
Acridine orange hydrochloride
, Basic Orange 14
, N,N,N’,N’-Tetramethyl-3,6-acridinediamine hydrochloride
, C.I. 46005
, 3,6-Bis(dimethylamino)acridine hydrochloride
, 3,6-Acridinediamine
, AO
ENZ-52405
89166-034EA
105
USD
InStock
89166-034
Acridine orange (chlorid salt) ≥95% (by HPLC), Ultrapure
Acridine orange (chlorid salt)
DNA dye
Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). Acridine Orange is also commonly used to non-specifically stain acidic organelles, such as lysosomes The dye is often used in epifluorescence microscopy. Wavelength Maxima: Excitation 502nm, Emission 525nm