Synonyms:
DAPI 2HCl
, 2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride
, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
, DAPI.2HCl
, 2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride
0215757410
0215757401
0215757405
0215757450
IC15757405EA
85.61
USD
InStock
IC15757405
IC15757410
IC15757401
76102-756
4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride), yellow powder
4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride)
4',6-Diamidino-2-phenylindole Dihydrochloride is a cell permeable fluorescent minor groove-binding probe for DNA.
It binds to double-stranded DNA by forming a stable complex which fluoresce. DAPI selectively forms complexes with A-T, A-U and I-C base pairs (preferably A-T rich DNA), having one molecule of dye for every 3 base-pairs. DAPI in aqueous solution has two absorbance maxima, at 349 nm and at 263 nm. It can be used as a vital dye to stain nuclei in mature living pollen grains in conjuction with fluroescein diacetate.
Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Slightly soluble in water (10 mg/mL - clear, yellow to yellow-green solution - heat or sonication may be required).