Synonyms:
NHS-Rhoda
, 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester
, TAMRA SE, 5 and 6 isomers
, NHS-Rhodamine
, TMR-SE
, 5(6)-TAMRA N-succinimidyl ester
46406
46406
PI46406EA
230.03
USD
InStock
PI46406
CAPI46406
5(6)-Carboxytetramethylrhodamine succinimidyl ester [5(6)-TAMRA SE] mixture of isomers fluorescent dye, Pierce™
5(6)-Carboxytetramethylrhodamine succinimidyl ester [5(6)-TAMRA SE]
Thermo Scientific NHS-Rhodamine is a high-performance derivative of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
- Amine specific labeling
- Label antibodies for use as immunofluorescent probes
- Label oligonucleotides for hybridization probes
- Detect proteins in gels and on western blots
- Excitation⁄Emission: 552⁄575 (nm)
NHS Rhodamine is an amine-reactive derivative of rhodamine dye that has wide-ranging application as antibody and other probe labels for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.
Thermo Scientific Pierce Rhodamine Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.
NHS-Rhodamine is activated with the N-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to TRITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling.
Caution: For Research Use Only. Not for use in diagnostic procedures. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20˚C.