Synonyms:
4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester
, Fluo-3 AM ester
, 1-[Amino-5-(2,7-dichloro-6-acetomethoxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid pentaacetoxy-methyl ester
50015
89139-184EA
299.26
USD
InStock
89139-184
FLUO 3/AM 1 mM in anhydrous DMSO fluorescent indicator, for calcium determination
FLUO 3/AM
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.
- Absorption/Emission For Fluo-3: 506 nm/526 nm (low or high [Ca2+] )
- Extinction Coefficient For Fluo-3: 86,000 M -1 cm -1 (506 nm)
- Cell permeability: Membrane permeant
- Orange red solid soluble in DMSO
- Store -20°C and protect from light, especially when in solution