Synonyms:
BCIP (5-bromo-4-chloro-3-indolylphosphate toluidine salt)
, TNBT (tetra nitro-blue tetrazolium chloride)
BCBT-100
BCBT-100
RLBCBT-100EA
141
USD
InStock
CABCBT-100
RLBCBT-100
BCIP/TNBT alkaline phosphatase substrate
BCIP/TNBT
BCIP/TNBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of TNBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
BCIP/TNBT Membrane Alkaline Phosphatase Substrate is a useful detection reagent for WB, IHC (immunohistochemistry), and in situ hybridisation.
Storage Condition: Store container at 4° C prior to opening. Protect BCIP/TNBT Membrane Alkaline Phosphatase Substrate from moisture and light.