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Mix-n-Stain™ CF® Dye Antibody Labeling Kits, Biotium

Supplier: Biotium

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89171-584EA 159.23 USD InStock
89171-584 89171-540 89171-582 89171-580 89411-618 76404-042 76404-044 76404-046 76221-502 89171-548 89171-546 76221-504 89171-544 89171-588 89171-586 89411-620 89171-542 76221-500 89171-552 89171-596 89171-594 89171-550 89171-592 76404-050 89171-590 76404-052 76404-054 76404-056 76404-058 76404-048 89171-518 89171-516 89171-558 89171-514 89171-512 89171-556 89171-598 89171-554 89171-562 10119-160 89171-560 75832-518 75832-636 75832-830 75832-832 75832-520 89171-528 89171-606 89171-526 10119-158 89171-604 89171-524 89171-568 89171-602 89171-522 89171-566 89171-600 89171-520 89171-564 89411-608 75832-808 89171-574 89171-530 89171-572 89171-570 89411-606 75832-806 10119-156 75832-526 75832-528 75832-642 75832-522 75832-524 76221-494 89427-122 76221-496 89427-124 89427-120 89171-538 89411-612 89171-536 89411-614 76221-498 89171-578 89171-534 89171-576 89171-532
Mix-n-Stain™ CF® Dye Antibody Labeling Kits, Biotium
Protein Linking and Labeling Systems
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

  • Label 5 to 20 µg, 20 to 50 µg, or 50 to 100 µg Ab
  • Choice of 32 CF® Dye colors
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification, 100% recovery
  • Compatible with BSA, gelatin, ascites

Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification.

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

These kits feature a choice of 32 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.

Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye.

Biotium now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG.

Delivery information: Kit components: Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed), 10X Mix-n-Stain™ reaction buffer, Lyophilized Reactive CF® Dye and Mix-n-Stain™ storage buffer.

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