Synonyms:
Iodoacetic acid N-succinimidyl Ester
, SIA
, N-(Iodoacetoxy)succinimide
, 2,5-Dioxopyrrolidin-1-yl 2-iodoacetate
22349
22349
PI22349EA
160.25
USD
InStock
CAPI22349
PI22349
SIA (N-Succinimidyl iodoacetate)
SIA (N-Succinimidyl iodoacetate)
Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
- Reactive groups: NHS ester and iodoacetyl
- Reactive toward: amino and sulfhydryl groups
- Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare to SIAB
- NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
- Iodoacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bond
- Non-cleavable
- Water-insoluble (dissolve first in DMF or DMSO)
Caution: For Research Use Only. Not for use in diagnostic procedures.