Synonyms:
3-Pyrrolidinesulphonic acid, 2,5-dioxo-1-[[1-oxo-6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]oxy]-, sodium salt (1:1)
, SODIUM 2,5-DIOXO-1-({6-[3-(PYRIDIN-2-YLDISULFANYL)PROPANAMIDO]HEXANOYL}OXY)PYRROLIDINE-3-SULFONATE
, SODIUM 2,5-DIOXO-1-({6-[3-(PYRIDIN-2-YLDIsulphANYL)PROPANAMIDO]HEXANOYL}OXY)PYRROLIDINE-3-sulphONATE
, 2,5-Dioxo-1-[[1-oxo-6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]oxy]-
, 3-Pyrrolidinesulfonic acid, 2,5-dioxo-1-[[1-oxo-6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]oxy]-, sodium salt (1:1)
21650
21650
PI21650EA
734.03
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InStock
PI21650
CAPI21650
Sulfo-LC-SPDP (Sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate) ≥90%, Pierce™
Sulfo-LC-SPDP (Sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.
- Reactive groups: NHS ester and pyridyldithiol
- Reactive towards: amino and sulfhydryl groups
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Water soluble, sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
- Cleavable crosslinker allows separation of crosslinked products
- Sulfo-LC-SPDP crosslinker is not membrane permeable, allowing cell surface labeling
- Compare to other varieties of SPDP-type reagents, including pegylated forms
Sulfo-LC-SPDP is Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.
Caution: For Research Use Only. Not for use in diagnostic procedures.