Synonyms:
Trimethylglycine
, TMG
V351104
76620-530EA
64.37
USD
InStock
76620-530
Betaine enhancer solution 5M
Betaine
Betaine enhancer is especially effective when used with high GC-rich regions or templates with a high degree of secondary structures. It has a decreasing effect on the primer melting temperature.
- Enhances amplification on GC-rich and difficult DNA targets
- Lowers the melting temperature
The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.
Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.
Caution: For Research Use Only. Not for use in diagnostic procedures.