Synonyms:
BCIG
, (2S,3R,4S,5R,6R)-2-((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
0215000105
0215000190
0215000150
0215000110
0215000183
0215000180
0215000191
IC15000105EA
49.65
USD
InStock
IC15000105
IC15000180
IC15000191
IC15000190
IC15000150
IC15000183
75875-614
X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside), white powder
X-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.
X-Gal is particularly useful in molecular biology applications to detect the activity of b-galactosidase which is frequently used as a reporter gene. Insertion of DNA into the lac Z region results in the loss of b-galactosidase activity. Lac+ bacterial colonies resulting from a-complementation will appear blue whereas bacterial colonies containing plasmid with DNA inserted in the lac Z region, will be incapable of a-complementation and will appear white.
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside is used as indigogenic substrate for b - galactosidase, for detection of b - galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.