Synonyms:
4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid
, 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid
, HEPES free acid
61-034-RM
61-034-RR
61-034-RO
61-034-RO
61-034-RR
61-034-RM
45000-694EA
267.62
USD
InStock
45000-694
CA45000-692
45000-692
CA45000-696
45000-696
CA45000-694
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid, powder acid-free, Corning®
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)
HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2
Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.