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Cloning Buffers

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Cloning Buffers

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.


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Description:   NEBuffer* Set, 4 vials of NEBuffers for restriction enzymes requiring unique buffers (2 x EcoRI, 2 x DpnII), Assure optimal (100%) activity, Concentration: 10X, Applications: Cloning Ligation, Storage: -20 deg C, Size: 1.25ml
Catalog Number: 76285-182
Supplier: New England Biolabs (NEB)



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Description:   Tris (tris[hydroxymethyl]aminoethane), 1M, Recipe: 121.14g Tris Base, Sterile Filtered: (0.20 uM), Storage: Room Temperature, pH: 7.4-7.6, Polycarbonate Carboy, For use as an electrophoresis buffer and to increase cell membrane permeability, Size: 1x10l
Catalog Number: 75800-962
Supplier: GROWCELLS



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Catalog Number: RLMB-057
Supplier: Rockland Immunochemical


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Description:   6.0 ml.
Catalog Number: 101227-460
Supplier: New England Biolabs (NEB)


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Description:   Tris (tris[hydroxymethyl]aminoethane), 1M, Recipe: 121.14g Tris Base, Sterile Filtered: (0.20 uM), Storage: Room Temperature, pH: 7.4-7.6, Polycarbonate Bottle, For use as an electrophoresis buffer and to increase cell membrane permeability, Size: 1x1l
Catalog Number: 75800-958
Supplier: GROWCELLS



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Description:   6.0 ml.
Catalog Number: 101227-458
Supplier: New England Biolabs (NEB)


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Description:   Tris (tris[hydroxymethyl]aminoethane), 1M, Recipe: 121.14g Tris Base, Sterile Filtered: (0.20 uM), Storage: Room Temperature, pH: 7.4-7.6, Sterile Cubitainer, For use as an electrophoresis buffer and to increase cell membrane permeability, Size: 1x10l
Catalog Number: 75800-964
Supplier: GROWCELLS



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Description:   TE (Tris EDTA), 1X, Recipe: 10ml 1M Tris pH 7.5, 2ml 0.5M EDTA pH 8, Sterile Filtered: 0.20uM, Storage: Room Temperature, Polycarbonate Bottle, Nuclease free, Used to solubilize DNA/RNA providing protection from degradation, electrophoresis & storage of nucleic acids, Size: 10l
Catalog Number: 75800-976
Supplier: GROWCELLS



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Catalog Number: 76486-070
Supplier: New England Biolabs (NEB)



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Catalog Number: 76559-396
Supplier: New England Biolabs (NEB)



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Description:   TE (Tris EDTA), 1X, Recipe: 10ml 1M Tris pH 7.5, 2ml 0.5M EDTA pH 8, Sterile Filtered: 0.20uM, Storage: Room Temperature, Polycarbonate Bottle, Nuclease free, Used to solubilize DNA/RNA providing protection from degradation, for electrophoresis & storage of nucleic acids, Size: 1l
Catalog Number: 75800-970
Supplier: GROWCELLS



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Description:   DNA Ligase Buffer, supplied with T3, T7, Hi-T4 and Salt-T4 DNA Ligases, At a 1X concentration, this reaction buffer ensures optimal activity of these DNA ligases, Provided as a 2X solution, Contains 2 vials of buffer, Storage: -20 deg C, Volume: 2ml
Catalog Number: 76423-398
Supplier: New England Biolabs (NEB)



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Description:   TE pH 8.0
Catalog Number: 10128-420
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   NEBNext End Repair Reaction Buffer
Catalog Number: 101710-270
Supplier: New England Biolabs (NEB)


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Catalog Number: RLMB-042
Supplier: Rockland Immunochemical


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Description:   6.0 ml.
Catalog Number: 101227-462
Supplier: New England Biolabs (NEB)


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