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Cloning Buffers

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Cloning Buffers

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.


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Description:   N.I.S.T traceable to SRM 919. Suitable as a titrant or testing standard.
Catalog Number: BDH7830-1
Supplier: VWR International



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Catalog Number: 97063-290
Supplier: VWR



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Catalog Number: 97062-624
Supplier: VWR



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MSDS SDS Certificate Certificates    
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Catalog Number: 97062-654
Supplier: VWR



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Catalog Number: 97062-656
Supplier: VWR



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MSDS SDS Certificate Certificates    
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Catalog Number: 97062-836
Supplier: VWR



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Catalog Number: 97062-834
Supplier: VWR



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Catalog Number: 97062-626
Supplier: VWR



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Catalog Number: 97064-348
Supplier: VWR



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MSDS SDS Certificate Certificates    
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Description:   6.0 ml.
Catalog Number: 101227-464
Supplier: New England Biolabs (NEB)


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Catalog Number: 76559-402
Supplier: New England Biolabs (NEB)



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Description:   NEBNext dA-Tailing Reaction Buffer
Catalog Number: 101710-272
Supplier: New England Biolabs (NEB)


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Description:   EDTA (0.5 M), pH 8.0
Catalog Number: 10128-444
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   TE (Tris EDTA), 1X, Recipe: 10ml 1M Tris pH 7.5, 2ml 0.5M EDTA pH 8, Sterile Filtered: 0.20uM, Storage: Room Temperature, Polycarbonate Bottle, Nuclease free, Used to solubilize DNA/RNA providing protection from degradation, for electrophoresis & storage of nucleic acids, Size: 2l
Catalog Number: 75800-972
Supplier: GROWCELLS



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Catalog Number: 76486-068
Supplier: New England Biolabs (NEB)



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Description:   EDTA (0.5 M), pH 8.0
Catalog Number: 10128-442
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   0.5 M EDTA, pH 8.0
Catalog Number: RLMB-014
Supplier: Rockland Immunochemical


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Description:   TE (Tris EDTA), 1X, Recipe: 10ml 1M Tris pH 7.5, 2ml 0.5M EDTA pH 8, Sterile Filtered: 0.20uM, Storage: Room Temperature, Polycarbonate Bottle, Nuclease free, Used to solubilize DNA/RNA providing protection from degradation, electrophoresis & storage of nucleic acids, Size: 10l
Catalog Number: 75800-974
Supplier: GROWCELLS



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Description:   TE pH 8.0
Catalog Number: 10128-418
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   TNE Buffer 500ml, (Special Production, 2-4wks for delivery), clear, colorless liquid, Contains 1.211 g/L TRIS, 5.844 g/L Sodium Chloride, 0.372 g/L EDTA Disodium Dihydrate, and pH to 7.9
Catalog Number: 10128-582
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   TE pH 7.4
Catalog Number: 10128-414
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   3.0 M Sodium Acetate, pH 5.2
Catalog Number: RLMB-016
Supplier: Rockland Immunochemical


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Description:   NEBNext Quick Ligation Reaction Buffer (5X)
Catalog Number: 101710-268
Supplier: New England Biolabs (NEB)


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Description:   6.0 ml.
Catalog Number: 101227-498
Supplier: New England Biolabs (NEB)


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Description:   EDTA (0.5 M), pH 8.0
Catalog Number: 10128-446
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   TE pH 8.0
Catalog Number: 10128-422
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   NEBNext* Adaptor Dilution Buffer, optimized for dilution of the NEBNext Adaptor to the appropriate concentration range for efficient ligation to end-repaired, dA-tailed DNA, size: 9.6 ml
Catalog Number: 76326-062
Supplier: New England Biolabs (NEB)



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Description:   TE pH 7.4
Catalog Number: 10128-412
Supplier: QUALITY BIOLOGICAL, INC.


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Catalog Number: RLMB-040
Supplier: Rockland Immunochemical


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Description:   Low-EDTA TE 1X pH 8.0 4x100ml solution consisting of 10mM Tris Ultrapure, pH 8.0, and 0.1mM EDTA. Solution is 0.2 um filtered.
Catalog Number: 10128-588
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   TE pH 7.5 1000ml, Clear colourless liquid, Tris base 1.21 g/L 10mM; disodium EDTA 2H2O 0.37 g/L 1mM; sufficient HCl to adjust to pH 7.5.
Catalog Number: 10128-586
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   TE pH 7.4
Catalog Number: 10128-416
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   NEBuffer* Set, 4 vials of NEBuffers for restriction enzymes requiring unique buffers (2 x EcoRI, 2 x DpnII), Assure optimal (100%) activity, Concentration: 10X, Applications: Cloning Ligation, Storage: -20 deg C, Size: 1.25ml
Catalog Number: 76285-182
Supplier: New England Biolabs (NEB)



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Description:   Tris (tris[hydroxymethyl]aminoethane), 1M, Recipe: 121.14g Tris Base, Sterile Filtered: (0.20 uM), Storage: Room Temperature, pH: 7.4-7.6, Polycarbonate Carboy, For use as an electrophoresis buffer and to increase cell membrane permeability, Size: 1x10l
Catalog Number: 75800-962
Supplier: GROWCELLS



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Catalog Number: RLMB-057
Supplier: Rockland Immunochemical


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Description:   6.0 ml.
Catalog Number: 101227-460
Supplier: New England Biolabs (NEB)


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Description:   Tris (tris[hydroxymethyl]aminoethane), 1M, Recipe: 121.14g Tris Base, Sterile Filtered: (0.20 uM), Storage: Room Temperature, pH: 7.4-7.6, Polycarbonate Bottle, For use as an electrophoresis buffer and to increase cell membrane permeability, Size: 1x1l
Catalog Number: 75800-958
Supplier: GROWCELLS



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Description:   6.0 ml.
Catalog Number: 101227-458
Supplier: New England Biolabs (NEB)


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Description:   Tris (tris[hydroxymethyl]aminoethane), 1M, Recipe: 121.14g Tris Base, Sterile Filtered: (0.20 uM), Storage: Room Temperature, pH: 7.4-7.6, Sterile Cubitainer, For use as an electrophoresis buffer and to increase cell membrane permeability, Size: 1x10l
Catalog Number: 75800-964
Supplier: GROWCELLS



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Description:   TE (Tris EDTA), 1X, Recipe: 10ml 1M Tris pH 7.5, 2ml 0.5M EDTA pH 8, Sterile Filtered: 0.20uM, Storage: Room Temperature, Polycarbonate Bottle, Nuclease free, Used to solubilize DNA/RNA providing protection from degradation, electrophoresis & storage of nucleic acids, Size: 10l
Catalog Number: 75800-976
Supplier: GROWCELLS



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Catalog Number: 76486-070
Supplier: New England Biolabs (NEB)



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Catalog Number: 76559-396
Supplier: New England Biolabs (NEB)



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Description:   TE (Tris EDTA), 1X, Recipe: 10ml 1M Tris pH 7.5, 2ml 0.5M EDTA pH 8, Sterile Filtered: 0.20uM, Storage: Room Temperature, Polycarbonate Bottle, Nuclease free, Used to solubilize DNA/RNA providing protection from degradation, for electrophoresis & storage of nucleic acids, Size: 1l
Catalog Number: 75800-970
Supplier: GROWCELLS



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Description:   DNA Ligase Buffer, supplied with T3, T7, Hi-T4 and Salt-T4 DNA Ligases, At a 1X concentration, this reaction buffer ensures optimal activity of these DNA ligases, Provided as a 2X solution, Contains 2 vials of buffer, Storage: -20 deg C, Volume: 2ml
Catalog Number: 76423-398
Supplier: New England Biolabs (NEB)



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Description:   TE pH 8.0
Catalog Number: 10128-420
Supplier: QUALITY BIOLOGICAL, INC.


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Description:   NEBNext End Repair Reaction Buffer
Catalog Number: 101710-270
Supplier: New England Biolabs (NEB)


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Catalog Number: RLMB-042
Supplier: Rockland Immunochemical


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Description:   6.0 ml.
Catalog Number: 101227-462
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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Description:   Tris (tris[hydroxymethyl]aminoethane), 1M, Recipe: 121.14g Tris Base, Sterile Filtered: (0.20 uM), Storage: Room Temperature, pH: 7.4-7.6, Polycarbonate Bottle, For use as an electrophoresis buffer and to increase cell membrane permeability, Size: 1x2l
Catalog Number: 75800-960
Supplier: GROWCELLS



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Description:   500ml. Colorless to pale yellow solution. RTECS AH4025000, CAS 60-00-4.
Catalog Number: 80058-050
Supplier: MilliporeSigma



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Description:   DNA Extraction Buffer
Catalog Number: EM-3700
Supplier: MilliporeSigma



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MSDS SDS Certificate Certificates    
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Catalog Number: PAV4233
Supplier: Promega Corporation



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MSDS SDS Certificate Certificates    
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Description:   250ml CAS: 1185-53-1, MDL: MFCD00012590 Liquid
Catalog Number: AAJ60080-AK
Supplier: Thermo Scientific Chemicals



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MSDS SDS Certificate Certificates    
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Description:   250ml CAS: 1185-53-1, MDL: MFCD00012590 Liquid
Catalog Number: AAJ62848-AK
Supplier: Thermo Scientific Chemicals



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MSDS SDS Certificate Certificates    
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Description:   100ml Liquid
Catalog Number: AAJ62907-AE
Supplier: Thermo Scientific Chemicals



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Description:   EDTA Buffer, pH 8.5, 1X reagent, Intended for heat-induced antigen retriever on FFPE tissue section prior to application of antibodies in IHC, Improves accessibility of antibodies to tissue antigens, Storage: 4-8 deg C, Volume: 100ml
Catalog Number: 103302-792
Supplier: Electron Microscopy Sciences



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Description:   10mM Tris-HCl, 0.1mM EDTA, pH 8.0. sterile solution. Size: 1000mL.
Catalog Number: 100219-884
Supplier: Teknova



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Description:   T4 RNA Ligase Reaction Buffer
Catalog Number: 102855-118
Supplier: New England Biolabs (NEB)


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Description:   250ml Liquid
Catalog Number: AAJ63293-AK
Supplier: Thermo Scientific Chemicals



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MSDS SDS Certificate Certificates    
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Description:   250ml Liquid
Catalog Number: AAJ62907-AK
Supplier: Thermo Scientific Chemicals



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MSDS SDS Certificate Certificates    
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Description:   250ml CAS: 127-08-2, MDL: MFCD00012458 Liquid
Catalog Number: AAJ62817-AK
Supplier: Thermo Scientific Chemicals



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MSDS SDS Certificate Certificates    
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Description:   pH 8.0.
Catalog Number: 82023-102
Supplier: G-Biosciences



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Description:   500ml Liquid
Catalog Number: AAJ63240-AP
Supplier: Thermo Scientific Chemicals



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MSDS SDS Certificate Certificates    
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Catalog Number: PAV6231
Supplier: Promega Corporation



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MSDS SDS Certificate Certificates    
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