Easy access to products and protocols for research use only in the identification of 2019-nCoV based on Centers for Disease Control and Prevention (CDC) recommendations
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Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Description:
STEADY-LUC Firefly HTS Kit Lyophilized2, Steady-Luc is a homogneous glow-type luminescence assay with signal half-life of about three hours for high-throughput screening in multiwell plates. Assay buffer is supplied in lyophilized form for convenient storage at -20C
Description:
STEADY-LUC Firefly HTS Kit Lyophilized1, Steady-Luc is a homogneous glow-type luminescence assay with signal half-life of about three hours for high-throughput screening in multiwell plates. Assay buffer is supplied in lyophilized form for convenient storage at -20C
Description:
provides a safe alternative to the traditional hazardous radioactive method for assaying DNA polymeraseactivity. The fluorescence-based method is highly senstive and simple, requiring only a fluorometer, microplate reader or realt-time PCR instrument to perform.
Description:
It Allows Temperature Control Of Non-Thermostable Enzymes Like Reverse Transcriptase; Similar To Hotstart Modification, Enzyme Activity Is Inactivated By Modification Of Lysine Residues With A Novel Chemical Modifier, And Restored By Heating To 45C Or Higher
Description:
provides a simple assay system for fast, highly sensitive detection of caspase-3 activity in HTS format. The (Ac-DEVD)2-R110 is completely hydrolyzed by the enzyme in two successive steps. The assay kit includes the Ac-DEVD-CHO inhibitor.
Description:
is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.
Description:
is a general substrate for caspases including caspases 3 & 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, & thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts.
Description:
is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.
Description:
Z-FR-R110 is a substrate for the cysteine proteases cathepsins B and L. The substrate has been used in flow cytometry studies of human monocytes and rat macrophages.
Description:
is a substrate for serine proteases, including cathepsins, kallikrein and plasmin. ex\lem of substrate= 330/390nm (weak fluorescence); ex\em of end product(AMC) = 342/441nm (strong fluorescence).
Description:
is a substrate for serine proteases, and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.
Description:
HotStart Polymerase Modification Kit provides an easy way to generate chemically-modified hot-start Taq polymerase. The modification is reversed after heating to 90 C. The kit is sufficient to modify up to 0.5 mg of enzyme.
Description:
provides a simple assay system for fast, highly sensitive detection of caspase-3 activity in HTS format. The (Ac-DEVD)2-R110 is completely hydrolyzed by the enzyme in two successive steps. The assay kit includes the Ac-DEVD-CHO inhibitor.
Description:
STEADY-LUC Firefly HTS Kit Lyophilized3, Steady-Luc is a homogneous glow-type luminescence assay with signal half-life of about three hours for high-throughput screening in multiwell plates. Assay buffer is supplied in lyophilized form for convenient storage at -20C
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