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Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labeled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.
Description:
MTSEA biotin-X selectively labels thiols in the cell surface under mild condition. The X-spacer should facilitate better biotin-avidin or streptavidin interaction than MTSEA biotin (90064).
Description:
is a highly selective thiol labeling probe. TS (thiosulfate) group is negatively charged and thus probes bearing a TS group are ideal for specifically labeling thiols exposed at the exterior cell surface because charged probes are less likely to enter cells.
Description:
Biotin BCN(Biocyclo[6.1.0]nonyne), an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1, 2, 3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells with copper in cell extracts
Description:
is used to covalently attach biotin to a molecule with an aldehyde or ketone group. The aminooxy group reacts with an aldehyde or ketone group to form a stable oxime linkage in an neutral aqueous condition. The reaction is fast and can be accelerated by adding aniline as a catalyst.
Description:
Like TS biotin ethylenediamine (92010) TS biotin-XX ethylenediamine is a highly selective thiol labeling probe. The XX spacer should facilitate better interaction between biotin and avidin or streptavidin.
Description:
Biotin-XX succinimidyl ester is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.
Description:
Biotin-PEO4-propionate succinimidyl ester is a water soluble biotin derivative that can be readily coupled to primary or secondary amines.
Description:
free base can be directly coupled to activated carboxylic acid groups without using an additional base. The X-spacer should facilitate better biotin-avidin or streptavidin interaction than biotin cadaverine free base (90079).
Description:
Biotin-12 SE is an amine-reactive biotin derivative with a long spacer group. This product can be conjugated to AA-dUTP or AA-UTP to make biotin-16-dUTP or biotin-16-UTP, two important products used in genomic studies.
Description:
Like TS biotin ethylenediamine (92010) TS biotin-X ethylenediamine is a highly selective thiol labeling probe. The X spacer should facilitate better interaction between biotin and avidin or streptavidin.
Description:
MTSEA biotin-XX selectively labels thiols in the cell surface under mild condition. The XX-spacer should facilitate better biotin-avidin or streptavidin interaction than MTSEA biotin (90064).
Description:
Biotin azide reacts with alkyne to form 1, 2, 3-triazole by copper(I) catalyzed 1, 3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with phosphine containing compound.
Description:
Biotin alkyne reacts with azide to form 1, 2, 3-triazole by copper(I) catalyzed 1, 3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form biotin labeled polymers.
Description:
Tyramide biotin-XX conjugate is used for tyramide signal amplification, The deposited biotin molecules can be detected with any of our CF labeled streptavidin conjugates.
Description:
can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.
Description:
can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.
Description:
The Biotin SE Protein Labeling Kit includes everything required to label proteins with biotin. Sufficient for three labelings of 1 mg protein each.