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Competent Cells

Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.


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Description:   1ml. Lysogens of bacteriophage lDE3, Suitable for production of protein from target genes cloned in pET vectors. pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme. Suppress basal expression of T7 RNA polymerase.
Catalog Number: 80601-680
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-832
Supplier: MilliporeSigma



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Description:   11 reaction kit. Includes Test Plasmid and SOC Medium. Widely used host for protein expression from pET recombinants. Suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in both lon and ompT proteases.
Catalog Number: 80030-834
Supplier: MilliporeSigma



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Description:   1ml. Host competent cells are isogenic with the lDE3 lysogens used for protein expression with pET, pETBlue*, and pTriEx* vectors, but lack a source of T7 RNA polymerase. Standard host used for initial cloning of pET, pETBlue and pTriEx vector constructs.
Catalog Number: 80601-676
Supplier: MilliporeSigma



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Catalog Number: 80109-914
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-324
Supplier: MilliporeSigma



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Catalog Number: 80109-906
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-050
Supplier: MilliporeSigma



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Catalog Number: 82601-916
Supplier: MilliporeSigma



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MSDS SDS Certificate Certificates    
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Description:   11 rxn. Efficiency>1x10(9th) cfu/ug. K-12 strain ideally suited as initial cloning host due to high transformation efficiency, blue-white screening capability & recA endA mutations. Single-use 50-µl volume. Include SOC Medium and Test Plasmid.
Catalog Number: 80601-638
Supplier: MilliporeSigma



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Catalog Number: 16003-950
Supplier: MilliporeSigma



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Catalog Number: 80109-916
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-328
Supplier: MilliporeSigma



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Catalog Number: 82601-996
Supplier: MilliporeSigma



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MSDS SDS Certificate Certificates    
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Catalog Number: 16003-990
Supplier: MilliporeSigma



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MSDS SDS Certificate Certificates    
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Description:   22 reaction kit. Includes Test Plasmid and SOC Medium. Widely used host for protein expression from pET recombinants. Suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in both lon and ompT proteases.
Catalog Number: 80030-836
Supplier: MilliporeSigma



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