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Enzymatic Methyl-seq (EM-seq™): A new option for 5mC and 5hmC identification

Webinar

Thursday, 29th October, 2020 
14.00 - 15.00 EST

Replay

Increase accuracy and sensitivity of methylation detection

Identification of 5mC and 5hmC in DNA increases insight into gene regulatory mechanisms. Bisulfite sequencing has been commonly used for this, but the chemical-based conversion damages and degrades DNA, introducing bias into the data. In this webinar we will discuss NEBNext® Enzymatic Methyl-seq (EM-seq™), an enzyme-based method that minimizes damage to DNA. This method enables longer insert sizes, lower duplication rates and reduced GC bias, resulting in more accurate and sensitive methylation detection.

Presented By:


Siva Chavadi


Siva Chavadi received his Ph.D. from the University of Hyderabad, India and his graduate and postdoctoral work utilized functional genomics-based tools to develop diagnostics, anti-bacterials and vaccines against TB.  Siva is now a Senior Field Application Scientist at New England Biolabs, focusing on next generation sequencing.