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The application of different sample preparation techniques to improve LC-MS based assay performance
The application of different sample preparation techniques to improve LC-MS based assay performanceWebinarReplayLearn New Targeted Workflows to Overcome Challenges in Biomolecule Characterization Liquid chromatography coupled with mass spectrometry (LC-MS) is a highly specific, sensitive and rapid technique that can be used for the determination of low-level target analytes in complex matrices, such as biological fluids, food products and also environmental samples. Injection of neat matrices onto the LC-MS system is typically not practical as this would result in a plethora of issues including, but not limited to:
These issues are highly undesirable, particularly in high throughput scenarios, as they result in significant reduction in chromatographic performance and column lifetime, more frequent instrument down-time and increased instrument maintenance and cleaning requirements, as well as impacting the quantitative robustness of the assay. This presentation will look at a range of sample preparation techniques, including protein precipitation, supported liquid extraction (SLE) and solid phase extraction (SPE), which will address the issues mentioned previously. For more information, please contact us at webinar@avantorsciences.com Presented by: Matt James Matt James is a Senior Research Scientist at Avantor Sciences. Matt gained his PhD at University of Bristol, working on the analysis of archaeological samples and also worked within the pharmaceutical industry for a year. Over the last 11 years, he has worked within the Technical Support, Applications and R&D teams within Hichrom, now part of Avantor. Recent focuses have been HPLC/UHPLC method transfer and translation, the use of solid core phases, development of next generation LC stationary phases and the development of LC methods for key applications, such as the analysis of nitrosamines by LC-MS/MS. Matt has co-authored seven peer reviewed articles and presented work at several conferences. |