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Successful separation of polar substances - RP? - HILIC? - Carbon?

Webinar

Thursday, 27th October, 2022
09:00 am to 10:00 am EST

Register

The analysis of polar substances is becoming increasingly important, but presents a particular challenge in HPLC.

The "universal column" C18 is only suitable to a limited extent for the HPLC separation of polar and hydrophilic substances. Often all kinds of "tricks" have to be used to achieve a good separation. Hydrophilic interaction chromatography (HILIC) is a good choice for the successful separation of polar compounds. But is HILIC the same as HILIC? A whole range of stationary phases are grouped under "HILIC".

This presents a particular hurdle in method development. Another option is the use of "Porous graphitic carbon (PGC) particles" for the separation of polar compounds. This phase opens up unique possibilities in HPLC. This presentation will clarify the differences as well as the advantages and limitations of the various stationary phases suitable for the separation of polar compounds in order to facilitate the selection of the most suitable phase for the individual separation problem.

For more information, please contact us at webinar@avantorsciences.com

Presented By:



Petra Lewits  

Petra has worked in Chromatography for more than 35 years. She started her career in separation science in 1983 using several chromatographic methods, including HPLC, TLC, GC and Solid Phase Extraction (SPE), for food control in a governmental institution lab. In 1986 she joined Merck´s pharmaceutical R&D and QC department (Merck KGaA in Darmstadt, Germany) were she developed HPLC methods for R&D and QC of pharmaceutical API´s and formulations as well as for diet convenience foods. From 1991 on she supported customers in different positions with technical and application related inquires in Europe as a sales specialist for HPLC instruments, technical support for Chromatography and complaint management. Since 2002 she is a Global Product Manager for Analytical Chromatography (HPLC). From 2014 she was focusing on Thin-Layer Chromatography (TLC). Since April 2018 Petra has the global responsibility for analytical HPLC columns at Merck.