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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   High efficiency blunt-end PCR cloning, features DNA topoisomerase I and Cre recombinase technologies. 20 reactions
Catalog Number: 99901-044
Supplier: Agilent Technologies



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Description:   Gibson Assembly* HiFi 1 Step Cloning Kit, Component: GA HiFi 1 Step Master Mix (2X),250 uL,GA Positive Control (2X), 25 uL (5 Control Reactions),Detection Range: 200 bp to 100 kb, up to 5 fragments in one reaction, Method: One-step, Quantity: 50 reactions
Catalog Number: 10820-614
Supplier: TELESIS BIO



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Description:   Quick Blunting Kit - 100 rxns
Catalog Number: 101417-940
Supplier: New England Biolabs (NEB)


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Catalog Number: 77506-682
Supplier: TELESIS BIO


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Catalog Number: 77461-254
Supplier: TELESIS BIO


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Catalog Number: 77461-270
Supplier: TELESIS BIO


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Description:   Packaging efficiency: 1e9 pfu/ug. Preferentially packages phage with large inserts; For genomic lambda and cosmid libraries Contains: Gigapack III XL packaging extract, control lambda DNA and control host strain 11 vials
Catalog Number: 99900-316
Supplier: Agilent Technologies



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Catalog Number: 77506-686
Supplier: TELESIS BIO


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Catalog Number: 77461-298
Supplier: TELESIS BIO


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Catalog Number: 77461-294
Supplier: TELESIS BIO


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Description:   Gibson Assembly* Ultra Master Mixes (2X)Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Range: 200 bp to 100 kb, Two step reactions to generate DNA with positive control, Quantity: 50 reactions
Catalog Number: 10820-652
Supplier: TELESIS BIO



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MSDS SDS    
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Description:   Kit contains: 400U DNA Ligase 10X reaction buffer 400 U
Catalog Number: 99902-306
Supplier: Agilent Technologies



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Catalog Number: 77463-574
Supplier: TELESIS BIO


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Description:   Gibson Assembly* Ultra Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, Two step reactions to generate DNA with positive control (2 reactions), Quantity: 10 reactions
Catalog Number: 10820-790
Supplier: TELESIS BIO



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MSDS SDS    
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Catalog Number: ST200552
Supplier: Agilent Technologies



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MSDS SDS    
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Description:   NEB* PCR Cloning Kit, contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. 20 reactions
Catalog Number: 102877-564
Supplier: New England Biolabs (NEB)


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