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Western Blotting Reagents

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Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Description:   (3,3',5,5'-Tetramethylbenzidine) 1.25 mM Produces a soluble blue end product read at 370 nm or 655 nm. Use of stop solution (equal volume 0.5 N HCl) enhances sensitivity 2-4 fold - the resulting yellow solution can be read at 450 nm.
Catalog Number: RLTMBE-100
Supplier: Rockland Immunochemical


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Description:   Clear to very light blue colored liquid. Produces a soluble blue end product read at 370nm or 655nm.
Catalog Number: RLTMBE-1000
Supplier: Rockland Immunochemical


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Description:   25 ml
Catalog Number: 95016-836
Supplier: Cytiva


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Description:   Sufficient for 1000 cm2 membrane (ECL Detection Reagents, Anti-Rabbit Conjugate, Anti-Mouse Conjugate and ECL Blocking Agent)
Catalog Number: 95038-570
Supplier: Cytiva



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Description:   36mg ECF substrate + 60mL detection buffer sufficient for 2500cm2 membrane
Catalog Number: 95038-296
Supplier: Cytiva


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Description:   ECL PLEX PK:CY3,CY5,Hybond -ECL
Catalog Number: 95040-084
Supplier: Cytiva


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Description:   HIGHDEF* DAB Chromogen/Substrate Set, Contains: HIGHDEF* DAB Chromogen, HIGHDEF* DAB Substrate Buffer, Applications: IHC, ISH (in situ hybridization), Long Term Storage: +4 deg C, Size: 200mL
Catalog Number: 75841-138
Supplier: Enzo Life Sciences



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Description:   100mg. Solid. Produces stable blue precipitate at reaction site thats soluble in dimethylformamide. The classical substrate used w/ b-galacto-sidase in current methods of molecular biology, microbiology, immunology
Catalog Number: RLXGAL-0100
Supplier: Rockland Immunochemical


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Description:   Is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). FemtoMax* for both western blotting and enzyme immunoassay (EIA) use.
Catalog Number: RLFEMTOMAX-110
Supplier: Rockland Immunochemical


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Description:   Sufficient reagents for 2500cm2 membrane detection (25 labelling reactions).
Catalog Number: 95038-288
Supplier: Cytiva


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Description:   Reagent, ECL western blot detection 200ml, entry level chemiluminescent detection reagent in the amersham ECL product family, used for confirmatory analysis and detection of high to medium levels of endogenous proteins.
Catalog Number: 10662-312
Supplier: Cytiva



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Description:   DeStreak Rehydration Solution, 5 x 3 ml. Improves reproducibility and quality of 2-D gels by preventing streaking.Eliminates extra spots caused by nonspecific oxidation of proteins.Stabilizes proteins throughout entire 2-D runs, ensuring simplified, well
Catalog Number: 95056-444
Supplier: Cytiva


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Description:   (3,3'-Diaminobenzidine) 0.0694 M A widely used substrate for both immunoblotting and immunohistochemical staining techniques. Produces an insoluble brown end product insoluble in, alcohol. A suspected carcinogen. Multi-component system.
Catalog Number: RLDAB-10
Supplier: Rockland Immunochemical


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Description:   (2,2'-Azino-bis[3-ethylbenzthiazoline -6-sulfonic acid]) 1.46 mM A safe, convenient and good, overall performing substrate for ELISA. ABTS develops an intense blue-green color measurable, between 405 nm and 410 nm. Suitable for kinetic and endpoint reactions.
Catalog Number: RLABTS-100
Supplier: Rockland Immunochemical


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Description:   (Caspase-8 Substrate II, Fluorogenic; Z-IETD-AFC; Z-Ile-Glu-Thr-Asp-AFC). A fluorogenic substrate for the detection of granzyme B and caspase-8 activity. Purity: >=97% by HPLC. Soluble in DMSO. FW: 821.8. Lyophilized solid, 1mg.
Catalog Number: 80051-942
Supplier: MilliporeSigma



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Description:   (Ac-DEVD-AFC; Ac-Asp-Glu-Val-Asp-AFC). Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme-B processed ICE-LAP3 and caspases -6,-7,-8, and -10. Purity: >=95% by HPLC. Soluble in DMSO. Off-white solid, 5mg.
Catalog Number: 80051-374
Supplier: MilliporeSigma



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