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Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.
Description:
E. cloni* 10G Chemically competent Cells (>1 x 108 cfu/¦g) are the best value in price and performance for everyday cloning applications. Packaged in two transformations per tube (DUOs) for convenience. 24 rxns.
Description:
ransforMax* EPI300* Chemically Competent E. Coli, Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA, >1 x 1010 cfu/ug pUC19 DNA, to regulate the copy number of Copy, Control Fosmid clones, Size: 10X50 ul
Description:
E. cloni* 10G ELITE Electrocompetent Cells (>2 x 1010 cfu/¦g) provide large numbers of transformants, even from hard-to-clone or limited DNA. Packaged in two transformations per tube (DUOs) for convenience. 12 rxns.
Description:
E. cloni* 10G Chemically competent Cells (>1 x 108 cfu/¦g) are the best value in price and performance for everyday cloning applications. Packaged in two transformations per tube (DUOs) for convenience. 12 rxns.
Description:
CopyCutter* EPI400* Electrocompetent E. Coli, significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins, Size: 10 x 50 ul
Description:
E. cloni* 10GF' ELITE Electrocompetent Cells (>2 x 1010 cfu/¦g) provide large numbers of transformants and production of ssDNA. Packaged in two transformations per tube (DUOs) for convenience. 24 rxns.
Description:
OverExpress* C43(DE3) Electrocomp Cells (? 1 x 1010 cfu/ug) are derivatives of BL21(DE3) strains that express toxic proteins. Packaged in one transformation per tube (SOLOs) for convenience. 12 rxns.
Description:
E. cloni* EXPRESS BL21(DE3) Electrocompetent Cells (? 5 x 109 cfu/ug) are perfect for everyday protein expression and offer the best value available from any supplier. Packaged in two transformations per tube (DUOs) for convenience. 12 rxns.
Description:
E. cloni* 10G SUPREME Electrocompetent Cells (>4 x 1010 cfu/¦g) have the highest transformation efficiency available anywhere for the most demanding cloning applications. Packaged in two transformations per tube (DUOs) for convenience. 12 rxns.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
OverExpress* C41(DE3) Electrocomp Cells (? 1 x 1010 cfu/ug) are derivatives of BL21(DE3) strains that express toxic proteins. Packaged in one transformation per tube (SOLOs) for convenience. 12 rxns.
Description:
1ml. Lysogens of bacteriophage lDE3, Suitable for production of protein from target genes cloned in pET vectors. pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme. Suppress basal expression of T7 RNA polymerase.
Description:
1ml. Host competent cells are isogenic with the lDE3 lysogens used for protein expression with pET, pETBlue*, and pTriEx* vectors, but lack a source of T7 RNA polymerase. Standard host used for initial cloning of pET, pETBlue and pTriEx vector constructs.