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Competent Cells

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Competent Cells

Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.


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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-048
Supplier: MilliporeSigma



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Description:   22 reaction kit with Test Plasmid and SOC Medium. Host for protein expression from pET recombinants. For production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in lon and ompT proteases. pLysS expresses T7 lysozyme.
Catalog Number: 80030-840
Supplier: MilliporeSigma



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Catalog Number: 16003-940
Supplier: MilliporeSigma



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Catalog Number: 95057-470
Supplier: Lucigen



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Description:   E. cloni* 10G ELITE Electrocompetent Cells (>2 x 1010 cfu/¦g) provide large numbers of transformants, even from hard-to-clone or limited DNA. Packaged in one transformation per tube (SOLOs) for convenience. 12 rxns.
Catalog Number: 89005-074
Supplier: Lucigen



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Catalog Number: 95057-476
Supplier: Lucigen



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Catalog Number: 89005-078
Supplier: Lucigen



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Catalog Number: 80509-776
Supplier: MilliporeSigma



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Description:   Derivative of E. coli that have been optimized for high efficiency transformation by heat shock, producing > 1 x 108 cfu/µg supercoiled pUC19 DNA. These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones. 4 plates.
Catalog Number: 95040-460
Supplier: Lucigen



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Description:   11 reaction kit. Includes Test Plasmid and SOC Medium. For general cloning purposes. Has extremely high transformation efficiency, blue/white screening of recombinants by lacZ alpha-complementation, endA and recA mutations, and F factor to support M13 growth.
Catalog Number: 80030-752
Supplier: MilliporeSigma



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Description:   TransforMax* EC100* Chemically Competent E. Coli, Includes: pUC19 Control DNA (100 pg/ul), Ideal for most cloning applications, cells provide very high transformation efficiency, Accepts large clones, Storage: -70 degree C, Size: 50ul/tube
Catalog Number: 75927-880
Supplier: Lucigen



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Description:   E. cloni* 10G SUPREME Electrocompetent Cells (>4 x 1010 cfu/¦g) have the highest transformation efficiency available anywhere for the most demanding cloning applications. Packaged in two transformations per tube (DUOs) for convenience. 24 rxns.
Catalog Number: 89002-672
Supplier: Lucigen



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Description:   .4ml size. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation.
Catalog Number: 80030-318
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-320
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-326
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-332
Supplier: MilliporeSigma



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