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Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.
Description:
E. cloni* 10G ELITE Electrocompetent Cells (>2 x 1010 cfu/¦g) provide large numbers of transformants, even from hard-to-clone or limited DNA. Packaged in two transformations per tube (DUOs) for convenience. 24 rxns.
Description:
22 reaction kit. Includes Test Plasmid and SOC Medium. Widely used host for protein expression from pET recombinants. Suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in both lon and ompT proteases.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
11 rxn. Efficiency>1x10(9th) cfu/ug. K-12 strain ideally suited as initial cloning host due to high transformation efficiency, blue-white screening capability & recA endA mutations. Single-use 50-µl volume. Include SOC Medium and Test Plasmid.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
CopyCutter* EPI400* Chemically Competent E. Coli, Each supplied with 1 ml, Induction Solution and 10 ul (100 pg/ul) pUC19 Control DNA, significantly lower the copy number, cloning of unstable DNA sequences, Size: 10 x 50ul
Description:
OverExpress* ChemComboPack* contains 3 reactions each of the four OverExpress chemically competent cell strains so that you can determine which strain is best for your application. Packaged in one transformation per tube (SOLOs) for convenience. 12 rxns.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).