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Competent Cells

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Competent Cells

Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.


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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-832
Supplier: MilliporeSigma



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Description:   1ml. Host competent cells are isogenic with the lDE3 lysogens used for protein expression with pET, pETBlue*, and pTriEx* vectors, but lack a source of T7 RNA polymerase. Standard host used for initial cloning of pET, pETBlue and pTriEx vector constructs.
Catalog Number: 80601-676
Supplier: MilliporeSigma



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Description:   1ml. Lysogens of bacteriophage lDE3, Suitable for production of protein from target genes cloned in pET vectors. pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme. Suppress basal expression of T7 RNA polymerase.
Catalog Number: 80601-680
Supplier: MilliporeSigma



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Catalog Number: 80109-906
Supplier: MilliporeSigma



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Description:   11 reaction kit. Includes Test Plasmid and SOC Medium. Widely used host for protein expression from pET recombinants. Suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in both lon and ompT proteases.
Catalog Number: 80030-834
Supplier: MilliporeSigma



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Description:   TransforMax* EC100D* pir+Electrocompetent E. Coli, rescue cloning of DNA transposed using the EZ-Tn5* <R6KYori/KAN-2> Insertion kit or EZ-Tn5* <R6KYori/KAN-2>TNP Transposome*, 15 rescue plasmid copies per cell, Size: 5 x 100 ul
Catalog Number: 75927-934
Supplier: Lucigen



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Catalog Number: 71003-028
Supplier: Lucigen



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Catalog Number: 89428-536
Supplier: Lucigen



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Description:   Versatile and useful in a wide variety of applications including routine cloning, subcloning and plasmid isolation with or without blue/white screening. 24 reactions.
Catalog Number: 95040-456
Supplier: Lucigen



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Description:   11 reaction kit with Test Plasmid and SOC Medium. Host for protein expression from pET recombinants. For production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in lon and ompT proteases. pLysS expresses T7 lysozyme.
Catalog Number: 80030-838
Supplier: MilliporeSigma



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Catalog Number: 16003-962
Supplier: MilliporeSigma



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Catalog Number: 16003-960
Supplier: MilliporeSigma



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Description:   22 rxn. Efficiency>1x10(9th) cfu/ug. K-12 strain ideally suited as initial cloning host due to high transformation efficiency, blue-white screening capability & recA endA mutations. Single-use 50-µl volume. Include SOC Medium and Test Plasmid.
Catalog Number: 80601-640
Supplier: MilliporeSigma



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Description:   NovaBlue is a general purpose strain featuring high transformation efficiency, blue/white screening, high plasmid yields, and single stranded plasmid rescue.
Catalog Number: 80030-482
Supplier: MilliporeSigma



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Catalog Number: 80509-790
Supplier: MilliporeSigma



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Catalog Number: 80509-788
Supplier: MilliporeSigma



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