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Competent Cells

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Competent Cells

Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.


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Description:   SHuffle* T7 Express Competent E. Coli, T7 expression, Protease deficient/B strain, Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm, Applications: E.coli Protein Expression, Size: 12 x 0.05 ml/tube
Catalog Number: 103391-806
Supplier: New England Biolabs (NEB)



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Description:   Subcloning Efficiency
Catalog Number: 200067-176
Supplier: New England Biolabs (NEB)


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Description:   NEB 5-alpha Competent E.coli (High Efficiency) - 96x50ul/tube (12 x 8- tube strips) Chemically competent E. Coli cells suitable for high efficiency transformation. This DH5A* derivative is supplied with pUC19 Transformation Control Plasmid and SOC Outgrowth Medium.
Catalog Number: 103218-958
Supplier: New England Biolabs (NEB)


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Catalog Number: ST200228
Supplier: Agilent Technologies



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Description:   BL21-CodonPlus (DE3)-RIPL Competent Cell, contain extra copies of rare E.Coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias & dramatically improve expression of sequence from other organisms, for protein expression, Size: 10x0.1mL
Catalog Number: 76193-370
Supplier: Agilent Technologies



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Description:   6x0.2 ml
Catalog Number: 101417-896
Supplier: New England Biolabs (NEB)


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Catalog Number: ST200157
Supplier: Agilent Technologies



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Catalog Number: ST200123
Supplier: Agilent Technologies



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Description:   Lemo21(DE3) Competent E. Coli, Antibiotic Resistance: cam, Applications: E.coli Protein Expression, IMPACT* Protein Expression, Membrane Protein Expression, Peptide Ligation, Toxic Protein Expression, Storage Temperature: -80 deg C, Size: 12 x 0.05 ml/tube
Catalog Number: 103391-800
Supplier: New England Biolabs (NEB)



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Catalog Number: ST200132
Supplier: Agilent Technologies



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Catalog Number: ST230194
Supplier: Agilent Technologies



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Description:   >=5e6 transformants/ug 5 x 0.2 mL
Catalog Number: 99900-346
Supplier: Agilent Technologies



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Description:   6x0.2 ml
Catalog Number: 101417-890
Supplier: New England Biolabs (NEB)


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Catalog Number: ST230350
Supplier: Agilent Technologies



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Description:   For use with cloning without the F' episome, >= 1e9 transformants/ug 5 x 0.2-ml
Catalog Number: 99900-328
Supplier: Agilent Technologies



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Catalog Number: ST200159
Supplier: Agilent Technologies



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Catalog Number: ST200234
Supplier: Agilent Technologies



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Description:   E. cloni* EXPRESS BL21(DE3) Electrocompetent Cells (? 5 x 109 cfu/ug) are perfect for everyday protein expression and offer the best value available from any supplier. Packaged in two transformations per tube (DUOs) for convenience. 24 rxns.
Catalog Number: 89005-022
Supplier: Lucigen



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Description:   E. cloni* 10G ELITE Electrocompetent Cells (>2 x 1010 cfu/¦g) provide large numbers of transformants, even from hard-to-clone or limited DNA. Packaged in two transformations per tube (DUOs) for convenience. 24 rxns.
Catalog Number: 89002-660
Supplier: Lucigen



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Description:   22 reaction kit. Includes Test Plasmid and SOC Medium. Widely used host for protein expression from pET recombinants. Suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in both lon and ompT proteases.
Catalog Number: 80030-836
Supplier: MilliporeSigma



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Catalog Number: 16003-990
Supplier: MilliporeSigma



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Catalog Number: 82601-996
Supplier: MilliporeSigma



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Catalog Number: 16003-934
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-050
Supplier: MilliporeSigma



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Catalog Number: 82601-916
Supplier: MilliporeSigma



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Description:   11 rxn. Efficiency>1x10(9th) cfu/ug. K-12 strain ideally suited as initial cloning host due to high transformation efficiency, blue-white screening capability & recA endA mutations. Single-use 50-µl volume. Include SOC Medium and Test Plasmid.
Catalog Number: 80601-638
Supplier: MilliporeSigma



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Description:   pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Catalog Number: 80030-328
Supplier: MilliporeSigma



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Catalog Number: 16003-950
Supplier: MilliporeSigma



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Catalog Number: 80109-916
Supplier: MilliporeSigma



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Description:   Recovery medium for cloning cells (8 x 12ml).
Catalog Number: 89047-690
Supplier: Lucigen



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Description:   CopyCutter* EPI400* Chemically Competent E. Coli, Each supplied with 1 ml, Induction Solution and 10 ul (100 pg/ul) pUC19 Control DNA, significantly lower the copy number, cloning of unstable DNA sequences, Size: 10 x 50ul
Catalog Number: 75927-876
Supplier: Lucigen



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Description:   OverExpress* ChemComboPack* contains 3 reactions each of the four OverExpress chemically competent cell strains so that you can determine which strain is best for your application. Packaged in one transformation per tube (SOLOs) for convenience. 12 rxns.
Catalog Number: 89027-150
Supplier: Lucigen



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