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Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.
Description:
CopyCutter* EPI400* Chemically Competent E. Coli, Each supplied with 1 ml, Induction Solution and 10 ul (100 pg/ul) pUC19 Control DNA, significantly lower the copy number, cloning of unstable DNA sequences, Size: 10 x 50ul
Description:
OverExpress* ChemComboPack* contains 3 reactions each of the four OverExpress chemically competent cell strains so that you can determine which strain is best for your application. Packaged in one transformation per tube (SOLOs) for convenience. 12 rxns.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
E. cloni* 10G CLASSIC Electrocompetent Cells (? 5 x 109 cfu/¦g) are the most economical choice for standard cloning and library construction. Packaged in six transformations per tube (SixPacks) for convenience. 48 rxns.
Description:
OverExpress* C43(DE3) pLysS Chemcomp Cells (? 1 x 109 cfu/ug) are derivatives of BL21(DE3) strains used to express toxic proteins. The pLysS offers the most control over protein expression. Packaged in one transformation per tube (SOLOs) for convenience. 12 rxns.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
0.4ml. Lysogens of bacteriophage lDE3, Suitable for production of protein from target genes cloned in pET vectors. pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme. Suppress basal expression of T7 RNA polymerase.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
0.4ml. Host competent cells that combine the key features of BL21 (& Tuner* derivative), Origami, and Rosetta strains to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm.
Description:
1ml. Host competent cells that combine the key features of BL21 (& Tuner* derivative), Origami, and Rosetta strains to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
NovaBlue is a general purpose strain featuring high transformation efficiency, blue/white screening, high plasmid yields, and single stranded plasmid rescue.
Description:
Store at -70[degree]C. JM109 and HB101 competent cells are available for convenient transformation. Promega offers two efficiencies for particular needs: High Efficiency at greater than 108cfu/ug and Subcloning Efficiency at greater than 107cfu/ug.