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Vectors, Plasmids and Libraries
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Vectors, Plasmids and Libraries
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In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
Description:
Store competent cells at -70[degree]C; store all other components at -20[degree]C. pGEM-T Vector Systems I and II are guaranteed for at least 6 months from date of purchase, when stored and handled properly.
Description:
Contains a mimimal promoter, response elements and mimimal promoter. Promoter-containing vectors that can be used as expression controls or as co-reporter vectors.
Description:
PGL4.52[Luc2P/Stat5Re/Hygro] Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
PGL4.82(Hrluc/Puro) Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
NanoLuc* (Nluc) luciferase (19.1kDa) enzyme. For luminescent reporting using furimazine to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity.
Description:
PGL4.18(Luc2P/Neo) Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
Store at -20[degree]C. The pCI-neo Mammalian Expression Vector is selectable for studying constitutive expression of genes in mammalian cells.
Description:
PGL4.19(Luc2Cp/Neo) Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
Store vector at -20[degree]C. The pSP64 Poly(A) Vector can be used as a standard cloning vector and also can be used for in vitro transcription from the SP6 promoter. The pSP64 Poly(A) Vector can be used to generate poly(A)+ transcripts in vitro.
Description:
Store vector DNA at -20[degree]C. Store glycerol stock of JM109 at -70[degree]C. The pRL family of Renilla luciferase (Rluc) control reporter vectors is designed for use with the Dual-Luciferase; Reporter Assay System.
Description:
Contains a mimimal promoter, response elements and mimimal promoter. Promoter-containing vectors that can be used as expression controls or as co-reporter vectors.
Description:
Contains a mimimal promoter, response elements and mimimal promoter. Promoter-containing vectors that can be used as expression controls or as co-reporter vectors.
Description:
PMAL-c6T Vector, Fusion to MBP significantly enhances the solubility and proper folding of proteins expressed in E. Coli, Vector includes His-tag and TEV Protease recognition site, allowing the protein of interest to be cleaved from MBP using TEV Protease, 200 ug/ml, Size: 50 ul
Description:
Store vector at -20[degree]C. The pSP72-pSP73 Vectors can be used as standard cloning vectors and also can be used for in vitro transcription from either strand using the SP6 or T7 promoter.
Description:
PGL4.33[Luc2P/Sre/Hygro] Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
PGL4.53[Luc2/Pgk] Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
PNLCoI3[luc2-P2A-NlucP/CMV/Hygro] Vector, Coincidence Reporter Vector, For: Features: Improve Confidence and Save Time, Employ Robust and Sensitive Reporter Pair, Efficiently Identify False Hits, Use Simple Detection Format, Storage: -20 deg C, size: 20 ug
Description:
PNLF1-NRF2 [CMV/neo] Vector, NRF2 Vector System enables simple quantification of intracellular NRF2 protein, contains a vector encoding NanoLuc* fused to the C-terminus of the HIF1A protein, Application: Hypoxia signaling, Compound screening, Storage: -20 deg C, size: 5 x 20 ug
Description:
PGL4.54[Luc2/Tk] Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
Contains a mimimal promoter, response elements and mimimal promoter. Promoter-containing vectors that can be used as expression controls or as co-reporter vectors.
Description:
PGL4.17(Luc2/Neo) Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
HaloTag* Control Vector, Rapid, Efficient and Covalent Capture of Binary and Higher Order Complexes Directly from Lysates, High Purity and Low Background, Ability to Fluorescently Label the Same Genetic Fusion, Compatibility with All Downstream Methods of Analysis, size : 20ug
Description:
Store competent cells at -70[degree]C; store all other components at -20[degree]C. pGEM-T Easy Vector Systems I and II are guaranteed for at least 6 months from the date of purchase when stored and handled properly.
Description:
NanoBRET* PPI Starter System, provide the vectors required to create NanoLuc* Luciferase and HaloTag* protein fusions to target proteins, Features: Understand Real Biology, Monitor Changes, See Improved Assay Performance, Scale Your Assays, Storage: -30 deg C to -10 deg C,
Description:
NanoLuc* (Nluc) luciferase (19.1kDa) enzyme. For luminescent reporting using furimazine to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity.
Description:
PLIVE* Vector Complete System, designed for high and prolonged expression of transgenes in the mouse liver. In addition to the pLIVE Vector, pLIVE-LacZ (encoding Beta-galactosidase) and pLIVE-SEAP are provided as positive controls.
Description:
Plive Vector/Seap Control, designed for high and prolonged expression of transgenes in the mouse liver. Expression of the SEAP (Secreted Embryonic Alkaline Phosphatase) gene from pLIVE-SEAP can be easily monitored using a quantitative assay of mouse serum, Size: 20ug
Description:
PFN21A Halotag Cmv Flexi Vector, designed for expression of N-terminal-tagged HaloTag* fusion proteins in mammalian cells, for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag* Ligands,, Size: 20ug
Description:
PF3K Wg (Bydv) Flexi Vector, simple, yet powerful, directional cloning method for protein-coding sequences, based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions, Size: 20ug
Description:
PGL4.83(Hrlucp/Puro)Vector, Signaling Pathway Analysis (Minimal Promoter-Driven) Firefly Luciferase, Optimized For Expression In Mammalian Cells, report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the PGL4 backbone, size: 20ug
Description:
Provides constitutive high-level protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate-early enhancer/promoter. The vector can be used for both transient and stable gene expression.
Description:
NanoLuc* (Nluc) luciferase (19.1kDa) enzyme. For luminescent reporting using furimazine to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity.
Description:
NanoLuc* (Nluc) luciferase (19.1kDa) enzyme. For luminescent reporting using furimazine to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity.
Description:
Vector offers synthetic firefly luciferase luc2 gene under the control of the strong constitutive CMV (cytomegalovirus) promoter. Vector demonstrates high expression levels in a variety of cell lines tested.
Description:
Contains a mimimal promoter, response elements and mimimal promoter. Promoter-containing vectors that can be used as expression controls or as co-reporter vectors.
Description:
PNLF1-HIF1A [CMV/neo] Vector, HIF1A Vector System enables simple quantification of intracellular HIF1A protein, contains a vector encoding NanoLuc* fused to the C-terminus of the HIF1A protein, Application: Hypoxia signaling, Compound screening, Storage: -20 deg C, size: 5 x 20 ug
Description:
Provides constitutive high-level protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate early enhancer/promoter. The vector can be used for both transient and stable gene expression.
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