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MilliporeSigma

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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.


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Description:   The 4X SDS Sample Buffer is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at 2X for most applications, Storage -20degreeC
Catalog Number: EM70607-3
Supplier: MilliporeSigma



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Description:   ( [3-(Cyclohexylamino)propanesulfonic acid]). White solid. Purity: >= 99% by titration. Heavy metals: <= 1 ppm. Soluble in H2O. Absorbance (100 mM, 260 nm): <0.05. pKa 10.4 at 20[degree] C. RTECS TZ6395000, CAS 1135-40-6, M.W. 221.3.
Catalog Number: 80057-968
Supplier: MilliporeSigma



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Description:   100ml. 1.0M solution of ULTROL* Grade Hepes Free Acid (Cat. No. 391338). Sterile-filtered. Purity: >99% by titration (dry basis). RTECS TL6809000, CAS 7365-45-9.
Catalog Number: 80058-192
Supplier: MilliporeSigma



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Description:   PhosphoSafe* Extraction Reagent, When phosphorylation state matters, Phosphorylation state preserved during extraction, Storage ≤ -70degreeC, includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, beta-glycerophosphate
Catalog Number: EM71296-4
Supplier: MilliporeSigma



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Description:   ( [3-(Cyclohexylamino)propanesulfonic acid]). White solid. Purity: >= 99% by titration. Heavy metals: <= 1 ppm. Soluble in H2O. Absorbance (100 mM, 260 nm): <0.05. pKa 10.4 at 20[degree] C. RTECS TZ6395000, CAS 1135-40-6, M.W. 221.3.
Catalog Number: 80057-970
Supplier: MilliporeSigma



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Description:   (Phosphate buffered saline tablets). Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 10 mM phosphate buffer, pH 7.4 at 25[degree] C. Note: 1 each = ten tablets.
Catalog Number: 80058-556
Supplier: MilliporeSigma



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Catalog Number: EM-8890
Supplier: MilliporeSigma



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MSDS SDS Certificate Certificates    
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Description:   100gm. 3-(N-Morpholino)propanesulfonic Acid. Crystalline solid. Contaminants: DNases, proteases, RNases: none detected. Absorbance (260 nm and 280 nm): <=0.05. Heavy metals: <=0.0005% as Pb. pKa 7.2 at 20°C. RTECS QE9104530, CAS 1132-61-2.
Catalog Number: 80503-004
Supplier: MilliporeSigma



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Description:   (Phosphate buffered saline-Polyoxyethylene Sorbitan Monolaurate tablets). Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% Polyoxyethylene Sorbitan Monolaurate, 10 mM phosphate buffer, pH 7.4 at 25[degree] C. Note: 1 each = ten tablets.
Catalog Number: 80058-558
Supplier: MilliporeSigma



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Description:   ( [tris(Hydroxymethyl)aminomethane]). White solid. Widely used as a biological buffer. Purity: >= 99% by titration (dry basis). Heavy metals: <1 ppm. Soluble in H2O. Absorbance (1.0 M, H2O, 260 nm): <0.05. pH (1.0 M, H2O): 9.5 - 11, pKa 8.3 at 20[degree] C.
Catalog Number: 80058-768
Supplier: MilliporeSigma



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Certificate Certificates    
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Description:   PhosphoSafe* Extraction Reagent, When phosphorylation state matters, Phosphorylation state preserved during extraction, Storage ≤ -70degreeC, includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, beta-glycerophosphate, and sodium pyrophosphate
Catalog Number: EM71296-3
Supplier: MilliporeSigma



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Description:   500ml. Colorless to pale yellow solution. RTECS AH4025000, CAS 60-00-4.
Catalog Number: 80058-050
Supplier: MilliporeSigma



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Catalog Number: 80058-782
Supplier: MilliporeSigma



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Description:   500ml. 1.0M solution of ULTROL* Grade Hepes Free Acid (Cat. No. 391338). Sterile-filtered. Purity: >99% by titration (dry basis). RTECS TL6809000, CAS 7365-45-9.
Catalog Number: 80058-194
Supplier: MilliporeSigma



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Catalog Number: 82604-016
Supplier: MilliporeSigma



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Description:   ( [tris(Hydroxymethyl)aminomethane]). White solid. Widely used as a biological buffer. Purity: >= 99% by titration (dry basis). Heavy metals: <1 ppm. Soluble in H2O. Absorbance (1.0 M, H2O, 260 nm): <0.05. pH (1.0 M, H2O): 9.5 - 11, pKa 8.3 at 20[degree] C.
Catalog Number: 80058-772
Supplier: MilliporeSigma



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