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Promega Corporation


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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Catalog Number: (PAP2301)

Supplier:  Promega Corporation
Description:   NM522 contains an F' episome, which is required for production of ssDNA and for blue/white color selection.
Catalog Number: (PAM1051)

Supplier:  Promega Corporation
Description:   T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.
Catalog Number: (PAC1263)

Supplier:  Promega Corporation
Description:   For ligation of DNA ends.
Supplier:  Promega Corporation
Description:   For ligation of DNA ends, mutagenesis and cDNA cloning.

Supplier:  Promega Corporation
Description:   The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.
Supplier:  Promega Corporation
Description:   T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.

Supplier:  Promega Corporation
Description:   HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone a...

Supplier:  Promega Corporation
Description:   Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a rib...
Catalog Number: (PAM1794)

Supplier:  Promega Corporation
Description:   For ligation of DNA ends, mutagenesis and cDNA cloning
Supplier:  Promega Corporation
Description:   The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.
Catalog Number: (PAP9751)

Supplier:  Promega Corporation
Description:   Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restrictio...
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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The original product is no longer available. The replacement shown is available.
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