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Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.
Description:
NovaBlue is a general purpose strain featuring high transformation efficiency, blue/white screening, high plasmid yields, and single stranded plasmid rescue.
Description:
E. cloni* 10G ELITE Electrocompetent Cells (>2 x 1010 cfu/¦g) provide large numbers of transformants, even from hard-to-clone or limited DNA. Packaged in one transformation per tube (SOLOs) for convenience. 12 rxns.
Description:
E. cloni* 10G SUPREME Electrocompetent Cells (>4 x 1010 cfu/¦g) have the highest transformation efficiency available anywhere for the most demanding cloning applications. Packaged in two transformations per tube (DUOs) for convenience. 24 rxns.
Description:
Derivative of E. coli that have been optimized for high efficiency transformation by heat shock, producing > 1 x 108 cfu/µg supercoiled pUC19 DNA. These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones. 4 plates.
Description:
TransforMax* EC100* Chemically Competent E. Coli, Includes: pUC19 Control DNA (100 pg/ul), Ideal for most cloning applications, cells provide very high transformation efficiency, Accepts large clones, Storage: -70 degree C, Size: 50ul/tube
Description:
11 reaction kit. Includes Test Plasmid and SOC Medium. For general cloning purposes. Has extremely high transformation efficiency, blue/white screening of recombinants by lacZ alpha-complementation, endA and recA mutations, and F factor to support M13 growth.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
.4ml size. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
Store at -70[degree]C. JM109 and HB101 competent cells are available for convenient transformation. Promega offers two efficiencies for particular needs: High Efficiency at greater than 108cfu/ug and Subcloning Efficiency at greater than 107cfu/ug.
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