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Biotium

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.


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Description:   Fluorescent dyes with an aminooxy group can be conjugated to aldehyde or ketone groups in biomolecules. Far red fluorescent CF633 is significantly brighter than Alexa Fluor 647 or other Cy5-like cyanine dyes and is far more photostable.
Catalog Number: 89410-798
Supplier: Biotium



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Description:   CF Dye Maleimide, conjugation: CF800, are thiol-reactive fluorescent dyes, Maleimides are commonly used to label proteins on free thiol groups, next-generation fluorescent dyes, which have combined advantages in brightness, photostability, size: 0.25 umol
Catalog Number: 75784-064
Supplier: Biotium



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Description:   DUTP conjugated to CF405S a blue fluorescent dye with good spectral compatibility with popular instruments.
Catalog Number: 89410-674
Supplier: Biotium



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Description:   Tyramide Amplification Kit, Conjugate: Biotin XX, with HRP Goat Anti-Mouse and CF Dye/Biotin Tyramide(HRP), is enzyme-mediated detection method that uses catalytic activity of horseradish peroxidase to generate high density lab
Catalog Number: 76221-426
Supplier: Biotium



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Description:   Mix-n-Stain FITC Antibody Labeling Kit allow you to label your antibody in 30 minutes without a purification step. Kit is for labeling 5-20 ug of antibody with the traditional green fluorescent dye FITC. For a brighter and more photostable green dye
Catalog Number: 10018-228
Supplier: Biotium



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Description:   has been used to label thiols to confer the metal-binding properties of the phenanthroline chelator. For example, complexing of the chelator with copper can be used to selectively cleave RNA or DNA in the presence of hydrogen peroxide.
Catalog Number: 89139-810
Supplier: Biotium



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Description:   labels cytoplasmic membranes with an orange-red fluorescent membrane dye DiI. In combination with other CellBrite membrane stains, multiple cell populations can be labeled with distinctive colors, which is useful for the study of cell-cell interactions.
Catalog Number: 89138-944
Supplier: Biotium



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Description:   Is a-bungarotoxin labeled with our superior red fluorescent dye CF555. Alpha-Bungarotoxin is a polypeptide snake toxin that has high affinity for the nicotinic receptor at the neuromuscular junction.
Catalog Number: 71004-296
Supplier: Biotium



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Description:   can be coupled with activated carboxylic acid or sulfonyl chloride. CF488A dye is a superior alternative to FITC (or fluorescein) due to its exceptional brightness and photostability.
Catalog Number: 89139-814
Supplier: Biotium



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Description:   Fluorescently Labeled Human Transferrin Is Used To Track Recycling Endosomes By Fluroescence Microscopy; Deep Red Fluorescent Cf594 Is Spectrally Similar To Alexafluor 594 And Texas Red, With Superior Brightness And Photostability
Catalog Number: 89427-102
Supplier: Biotium



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Catalog Number: 97065-706
Supplier: Biotium



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Description:   5X concentrated binding buffer for Annexin V staining of apoptotic cells.
Catalog Number: 89411-114
Supplier: Biotium



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Description:   CF dye-dUTP, can be used for TUNEL assay, Conjugate: CF488A, or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications, is an orange fluorescent dye with an excitation spectrum optimally 543
Catalog Number: 76221-636
Supplier: Biotium



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Catalog Number: 76645-762
Supplier: Biotium



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Description:   Wheat germ agglutinin (WGA) conjugated to CF405S a blue fluorescent dye with good spectral compatibility with popular instruments. Fluorescent WGA conjugates can be used to stain glycoproteins containing sialic acid and N-acetylglucosamine moieties.
Catalog Number: 89410-826
Supplier: Biotium



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Description:   HRP WGA Wheat Germ Agglutinin (WGA) Conjugates
Catalog Number: 75845-664
Supplier: Biotium



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Description:   Mix-n-Stain* Antibody Labeling Kit, Dye: CF820, Ex/Em: 822/835 nm, Less than 30 second of handson time, 30 minutes total reaction time, No purification of the product is required, for 100% recovery, Compatible with common antibody stabilizers, size: 50-100ug
Catalog Number: 76404-058
Supplier: Biotium



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Description:   Streptavidin, HRP conjugate
Catalog Number: 10119-146
Supplier: Biotium



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Description:   The water-soluble form of D-luciferin. D-Luciferin is a substrate for the enzyme luciferase. Luciferase is encoded by the luc gene, which is widely used as a reporter gene in a variety of cells.
Catalog Number: 89138-286
Supplier: Biotium



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Description:   labeled with the CF488A dye, one of an outstanding series of CF dyes we have developed. The conjugate is useful for staining phosphatidylserine (PS), which is translocated from the inner to the outer leaflet of the plasma membrane during apoptosis.
Catalog Number: 89138-858
Supplier: Biotium



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Description:   Is a polypeptide snake toxin that binds to the nicotinic acetylcholine receptor found at the neuromuscular junction with high affinity. CF680R dye is spectrally similar to Cy5.5, Alexa Fluor 680, DyLight 680 and IRDye 680.
Catalog Number: 71004-006
Supplier: Biotium



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Description:   is a green fluorescent membrane dye which diffuses much faster than dio in cell membranes. dia and dii (60010) have been used together for two color membrane staining.
Catalog Number: 89139-304
Supplier: Biotium



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Description:   biotium developed neuro-dii as a replacement for dii (60010), neuro-dii and dii have nearly identical absorption and emission spectra, but the former has structural features that make the probe diffuse faster than dii on cell membranes and may also result in a more stable labeling.
Catalog Number: 89139-310
Supplier: Biotium



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Description:   is a synthetic derivative of coelenterazine, but the luminescence intensity of its aequorin complex is more than 10 times higher than that of the aequorin formed from native coelenterazine.
Catalog Number: 89138-298
Supplier: Biotium



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Description:   can be coupled with activated carboxylic acid or sulfonyl chloride. CF647 absorbs at 650 nm and emits at 674 nm. The dye is spectrally similar to Cy5 and Alexa Fluor 647.
Catalog Number: 89139-824
Supplier: Biotium



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Description:   Fluorescently Labeled Human Transferrin Is Used To Track Recycling Endosomes By Fluroescence Microscopy; Far Red/Near-Infrared Fluorescent Cf680R (Ex/Em 680/701 Nm) Is Spectrally Similar To Alexafluor 680, With Superior Photostability
Catalog Number: 89427-106
Supplier: Biotium



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Description:   Contains an outstanding far-red fluorescent CF640R dye (Abs/Em 642/662) and revolutionary labeling reagents for labeling antibodies. These novel dyes and labeling kits allow rapid preparation of 50-100ug of fluorescently labeled antibodies in just 30 min.
Catalog Number: 89171-528
Supplier: Biotium



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Description:   is a luminescent substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator measuring a broad concentration range of calcium from ~0.1 uM to >100 uM. Renilla luciferase has been widely used as a repoter protein.
Catalog Number: 89138-292
Supplier: Biotium



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Description:   Contains The Bright Orange Fluorescent Cf532 Dye (Abs/Em 527/558 Nm) And Labeling Reagents For Rapid Labeling Of 5-20 Ug Antibody In Only 30 Min, With No Separation Of Free Dye After Labeling; One Labeling Per Kit
Catalog Number: 89427-120
Supplier: Biotium



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Description:   Tyramide Amplification Kit, Conjugate: Biotin XX, with HRP Goat Anti-Rabbit and CF Dye/Biotin Tyramide, Conjugate: Streptavidin, is enzyme-mediated detection method uses catalytic activity of horseradish peroxidase to generate
Catalog Number: 76221-430
Supplier: Biotium



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Description:   CF660C phalloidin conjugate is the mushroom toxin phalloidin labeled with our far-red fluorescent dye CF660C for staining F-actin. CF660C is several fold brighter and significantly more photostable than Alexa Fluor 660.
Catalog Number: 10018-144
Supplier: Biotium



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Description:   Are Used For Highly Specific Staining Of F-Actin; Far Red Fluorescent Cf*660C (Ex/Em 667/685) Has Excellent Brightness And Photostability Compared To Spectrally Similar Dyes
Catalog Number: 89427-152
Supplier: Biotium



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Description:   binds to F-actin with nanomolar affinity. The red fluorescent phalloidin is typically applied to fixed and permeabilized cells but can also be loaded into live cells via cationic liposomes.
Catalog Number: 89138-124
Supplier: Biotium



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Catalog Number: 75784-074
Supplier: Biotium



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Description:   is the mushroom toxin phalloidin labeled with our superior far-red fluorescent dye CF640R. Phalloidin conjugates are excellent tools for cytoskeletal studies. CF640R dye is brighter and far more photostable than Alexa Fluor 647 when excited by the 633 or 635 nm laser.
Catalog Number: 89138-146
Supplier: Biotium



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Description:   comprises our superior near-IR amine-reactive dye CF680 SE and other components necessary for carrying out antibody labeling and purification. CF680 is significantly brighter and more stable than similar dyes such as Alexa Fluor 680, Cy5.5, DyLight 680 amd IRDye 680.
Catalog Number: 89139-846
Supplier: Biotium



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Description:   Contains an outstanding orange-red fluorescent CF555 dye (Abs/Em 555/565) and revolutionary labeling reagents for labeling antibodies. These novel dyes and labeling kits allow rapid preparation of 20-50ug of fluorescently labeled antibodies in just 30 min.
Catalog Number: 89171-552
Supplier: Biotium



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Description:   Fluorescent dyes with a hydrazide group can be conjugated to aldehyde and ketone groups in biomolecules. CF350 is a blue fluorescent dye spectrally similar to AMCA but with brighter fluorescence.
Catalog Number: 89410-812
Supplier: Biotium



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Description:   Contains an outstanding blue fluorescent CF405S dye (Abs/Em 404/431) and revolutionary labeling reagents for labeling antibodies. These novel dyes and labeling kits allow rapid preparation of 5-20ug of fluorescently labeled antibodies in just 30 min.
Catalog Number: 89171-578
Supplier: Biotium



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Description:   Label 5-20 ug of antibody with biotin in as little as 30 minutes. One labeling.
Catalog Number: 89411-616
Supplier: Biotium



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Description:   CF568 alkyne reacts with azide to form 1, 2, 3-triazole by copper(I) catalyzed 1, 3-dipolar Huisgen cycloaddition. Monomer to copolymerize with other monomers to form fluorescent polymers. Antibody conjugates of the dye are brighter than the spectrally similar Alexa Fluor 568.
Catalog Number: 10018-398
Supplier: Biotium



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Description:   Mix-n-Stain* Maxi Antibody Labeling Kit, Dye: CF405S, Ex/Em: 404/431nm, No purification required after labeling, Reaction takes 30 min with minimal handson time, Kit Component: Reaction Buffer, Vial of premeasured lyophilized dye, Quenching Buffer, Size: 1mg
Catalog Number: 76404-034
Supplier: Biotium



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Description:   CF dye dCTP, Conjugate: CF647, can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting application, dCTP conjugates, has similar brightness and photostability as Alexa Fluor 555, Supplied in lyoph
Catalog Number: 76221-640
Supplier: Biotium



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Description:   This labeling kit comprises our superior near-IR amine-reactive dye CF790 SE and other components necessary for carrying out antibody labeling and purification for whole animal near-infrared imaging.
Catalog Number: 89411-570
Supplier: Biotium



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Description:   CF550R Tyramide, Excitation/emission at 551/577 nm, can be used for tyramide signal amplification (TSA), Red fluorescent dye, Detection sensitivity of over 100-fold, Enables multiplex multicolor detection, with advantages in brightness, photostability, and specificity, Siize: 500ug
Catalog Number: 76423-360
Supplier: Biotium



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Description:   A derivative of BCIP. Unlike BCIP that yields a blue precipitating product, BCIP Red produces a red (max ~ 565 nm) colored precipitate.
Catalog Number: 89138-194
Supplier: Biotium



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Description:   CF555-dCTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting applications. CF555 has similar brightness and photostability as Alexa Fluor 555. Supplied in lyophilized form.
Catalog Number: 10098-600
Supplier: Biotium



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Description:   A derivative of BCIP. Unlike BCIP that yields a blue precipitating product, BCIP Pink produces a pink colored (max ~ 540 nm) precipitate. Protocol for using this product is similar to that of BCIP.
Catalog Number: 89138-198
Supplier: Biotium



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Description:   is one of the most widely used UV-excitable, blue fluorescent dye. MTS-AMCA readily labels a thiol with AMCA.
Catalog Number: 89139-762
Supplier: Biotium



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Description:   is a fast-reacting and highly selective thiol-reactive label that permits one to attach the popular fluorescent dye carboxytetramethylrhodamine to a thiol group.
Catalog Number: 89139-756
Supplier: Biotium



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Description:   reacts with aldehydes or ketones to a stable hydrazone compound under mild conditions. Similar to ARP (90073), aminooxy-5(6)-ROX can be used to label abasic sites in damaged DNA.
Catalog Number: 89139-708
Supplier: Biotium



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Description:   Cf532 Maleimide Is An Orange Fluorescent Dye With A Maleimide Reactive Group, Which Can Be Used To Label A Target Molecule With A Thiol Group; Cf532 Has An Excitation Spectrum Optimally Matching The 532 Nm Laser Line, And Protein Conjugates Of The Dye Are Much Brighter.
Catalog Number: 89427-114
Supplier: Biotium



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Description:   The water-soluble form of D-luciferin. D-Luciferin is a substrate for the enzyme luciferase. Luciferase is encoded by the luc gene, which is widely used as a reporter gene in a variety of cells.
Catalog Number: 89138-282
Supplier: Biotium



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Description:   CF Dye SE Protein Labeling Kits include everything required to label proteins with bright and photostable fluorescent CF Dyes. Sufficient for three labelings of 1 mg protein each. Far red CF640R is highly fluorescent and is significantly more photostable.
Catalog Number: 89411-600
Supplier: Biotium



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Description:   The amine-reactive quencher CFQ520 succinimidyl ester has a broad and intense absorption (approximately520 nm) but no fluorescence making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.
Catalog Number: 89410-774
Supplier: Biotium



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Description:   Mix-n-Stain CF430 Antibody Labeling Kit, labeling an antibody with biotin, CF*430 (abs/em=428 nm/498 nm), excited by violet laser (405 nm) and detected at AmCyan channel in flow. Size: 50-100 ug labeling
Catalog Number: 75832-518
Supplier: Biotium



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Description:   is uniformly dissolved in vegetable oil in a formulation optimized for microinjection. carbocyanine microinjection technique has been widely used for fate mapping and cell lineage studies and for the determination of spatial organization and connectivity patterns of central nervous systems.
Catalog Number: 89139-314
Supplier: Biotium



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Description:   MTS-5-TAMRA (MTS 5-carboxytetramethylrhodamine) is a fast-reacting and highly selective thiol-reactive label that permits one to attach the popular fluorescent dye carboxyttetramethylrhodamine to a thiol group.
Catalog Number: 89410-784
Supplier: Biotium



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Description:   Mix-n-Stain Small Lig& Labeling Kits are designed for rapid labeling of ligands or substrates including SNAP-tag, CLIP-tag and HaloTag ligands with an aliphatic amine; for labeling a small lig& with blue fluorescent CF405M for surface staining of live cells
Catalog Number: 10001-686
Supplier: Biotium



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Description:   Tyramide amplification is an enzyme-mediated detection method that uses the catalytic activity of horseradidsh peroxidase (HRP) to generate high density labeling of a target prostein. Contains HRP goat anti-rabbit with fa
Catalog Number: 76221-798
Supplier: Biotium



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Description:   CF Dye NanoBody Labeling Kit, optimized for labeling nanobodies with bright and photostable CF dyes in 30 minutes with no purification. Kits tolerate common stabilizers, including BSA. Far-red/near-IR CF680R dye has Ex/Em at 680/701 nm. One labeling of 20-50 ug nanobody per kit
Catalog Number: 76423-396
Supplier: Biotium



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Description:   CF660DI hydrazide could be used to react with aldehyde or ketone groups from protein carbonylation; CF660DI hydrazide, CF488DI hydrazide (92166) and CF555DI hydrazide (92164) are designed to have matching charge and molecular weight for use in 2D DIGE platform, 1mg
Catalog Number: 10001-772
Supplier: Biotium



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Description:   Tyramide Amplification Kit, Conjugate: CF543, with HRP Goat Anti-Mouse and CF Dye/Biotin Tyramide (HRP), is an enzyme-mediated detection method that uses catalytic activity of horseradish peroxidase to generate high density lab
Catalog Number: 76221-402
Supplier: Biotium



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Description:   CF660R-dCTP used to synthesize fluorescently-labeled DNA probes for in-situ hybridization, microarray or blotting techniques; CF60R (Ex/Em 663/682 nm) is a bright and exceptionally photostable dye in the borderline spectral region between far-red and near-infrared
Catalog Number: 10001-646
Supplier: Biotium



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