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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   NEBuilder HiFi DNA Assembly Bundle for Large Fragments - 20 reactions DNA assembly system for seamless joining of of single-stranded oligonucleotides or multiple DNA fragments, regardless of length or end compatibility
Catalog Number: 103218-906
Supplier: New England Biolabs (NEB)


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Description:   Salt-T4* DNA Ligase, Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids, Salt-T4 DNA Ligase seals nicks for these DNA substrates, Size: 20000 units
Catalog Number: 76407-346
Supplier: New England Biolabs (NEB)



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Description:   T4 RNA Ligase 2, truncated KQ
Catalog Number: 102715-908
Supplier: New England Biolabs (NEB)


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Description:   Applications: Synthesis of fusion protein in vivo using pGEMEX recombinant plasmids. Bacterial strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase.
Catalog Number: PAP9801
Supplier: Promega Corporation



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Catalog Number: 77025-080
Supplier: New England Biolabs (NEB)


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Description:   9 Degrees North DNA Ligase - 2,500 units
Catalog Number: 200064-420
Supplier: New England Biolabs (NEB)


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Description:   NEBNext Ultra Ligation Module
Catalog Number: 102855-130
Supplier: New England Biolabs (NEB)


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Description:   20,000 units.
Catalog Number: 101228-180
Supplier: New England Biolabs (NEB)


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MSDS SDS Certificate Certificates    
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Description:   100,000 units.
Catalog Number: 101228-176
Supplier: New England Biolabs (NEB)


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Certificate Certificates    
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Description:   150 reactions, Ligase
Catalog Number: 101227-654
Supplier: New England Biolabs (NEB)


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Description:   2,000 units.
Catalog Number: 101228-154
Supplier: New England Biolabs (NEB)


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Description:   T7 DNA Ligase
Catalog Number: 102500-172
Supplier: New England Biolabs (NEB)


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Description:   1,000 units.
Catalog Number: 101228-190
Supplier: New England Biolabs (NEB)


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Description:   500 units
Catalog Number: 101417-960
Supplier: New England Biolabs (NEB)


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Description:   T4 RNA Ligase 2, truncated KQ
Catalog Number: 102715-910
Supplier: New England Biolabs (NEB)


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Description:   Applications: Production of ssDNA. NM522 is supplied with the pGEM Single Strand System for the production of cloned inserts as single-stranded DNA (ssDNA).
Catalog Number: PAP2301
Supplier: Promega Corporation



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Description:   Gibson Assembly Master Mix
Catalog Number: 102500-054
Supplier: New England Biolabs (NEB)


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Description:   SplintR* Ligase, also known as PBCV-1 DNA Ligase or Chlorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand, 6, 250 units
Catalog Number: 102877-584
Supplier: New England Biolabs (NEB)


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Catalog Number: 76538-670
Supplier: New England Biolabs (NEB)



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Description:   T4 rna ligase 2, truncated k227q - 2,000 units
Catalog Number: 101640-958
Supplier: New England Biolabs (NEB)


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Catalog Number: 76717-036
Supplier: New England Biolabs (NEB)


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Catalog Number: 76717-054
Supplier: New England Biolabs (NEB)


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Description:   Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Catalog Number: 101386-088
Supplier: New England Biolabs (NEB)


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Catalog Number: PAM8225
Supplier: Promega Corporation



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Certificate Certificates    
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Catalog Number: PAC8820
Supplier: Promega Corporation



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Description:   Provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8), 100mM MgCl2, 100mM DTT, and 10mM ATP. This buffer should be stored frozen at -20[degree]C in small aliquots to preserve the ATP.
Catalog Number: PAM1804
Supplier: Promega Corporation



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Catalog Number: PAC1263
Supplier: Promega Corporation



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Description:   Salt-T4* DNA Ligase, Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids, Salt-T4 DNA Ligase seals nicks for these DNA substrates, Size: 100000 units
Catalog Number: 76407-348
Supplier: New England Biolabs (NEB)



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Description:   NEBuilder* HiFi DNA Assembly Master Mix, 10 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Catalog Number: 102877-804
Supplier: New England Biolabs (NEB)


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MSDS SDS Certificate Certificates    
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Description:   100,000 units, high concentration
Catalog Number: 101228-178
Supplier: New England Biolabs (NEB)


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Description:   T3 DNA Ligase
Catalog Number: 102500-170
Supplier: New England Biolabs (NEB)


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Description:   Modifying enzyme. For ligation, labeling, cloning. Concentration: 9-12 units/uL. Size: 500 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information (PPI) insert. 24-month lifetime.
Catalog Number: PAM1051
Supplier: Promega Corporation



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