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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Description:
Applications: Synthesis of fusion protein in vivo using pGEMEX recombinant plasmids. Bacterial strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase.
Description:
NEBuilder* HiFi DNA Assembly Master Mix, 10 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Description:
Modifying enzyme. For ligation, labeling, cloning. Concentration: 9-12 units/uL. Size: 500 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information (PPI) insert. 24-month lifetime.
Description:
NEBuilder* HiFi DNA Assembly Master Mix, 250 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Description:
Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Description:
RtcB Ligase, Concentration 15 uM, Source: RtcB Ligase is expressed as a His-tagged fusion in E. Coli single stranded RNA with a 3'-phospate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl (1,2). Ligation requires both GTP and MnCl2, Size: 25 reactions
Description:
Taq DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing the cloned Taq DNA ligase gene from Thermus aqauticus HB8, Product Includes: Taq DNA Ligase, 10X Taq DNA Ligase Buffer with NAD+, Size: 10, 000 units (250 µl)
Description:
ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 50 reactions
Description:
ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 100reactions
Description:
ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 10 reactions
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