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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Description:
NEB* Golden Gate Assembly kit, Updated to include BsaI-HFv2, Seamless cloning -no scar, plasmid with T7/SP6 promoters, Efficient with regions with high GC content and areas of repeats, Compatible w/ broad range of fragment sizes < 100 bp to > 15 kb, 20 rxns
Description:
Gibson Assembly* Ultra Master Mixes (2X) Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, Two step reactions to generate DNA, Quantity: 10 reactions
Description:
DNA Ligase T4 Molecular Biology Grade, Store vial at -20degree C prior to opening, Application Note: One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37degree C, 100units
Description:
Gibson Assembly* HiFi 1 Step Master Mix (2X) Cloning Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, up to 5 fragments in one reaction, One-step, 50 reactions
Description:
Gibson Assembly* Ultra Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, Two step reactions to generate DNA, Positive control: 5 reactions, Quantity: 50 reactions
Description:
Gibson Assembly* HiFi 1 Step Cloning Kit, Component: GA HiFi 1 Step Master Mix (2X),250 uL,GA Positive Control (2X), 25 uL (5 Control Reactions),Detection Range: 200 bp to 100 kb, up to 5 fragments in one reaction, Method: One-step, Quantity: 50 reactions
Description:
Gibson Assembly* Ultra Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, Two step reactions to generate DNA with positive control, Quantity: 5 reactions (no control)
Description:
Packaging efficiency: 1e9 pfu/ug. Preferentially packages phage with large inserts; For genomic lambda and cosmid libraries Contains: Gigapack III XL packaging extract, control lambda DNA and control host strain 11 vials
Description:
Gibson Assembly* Ultra Master Mixes (2X)Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Range: 200 bp to 100 kb, Two step reactions to generate DNA with positive control, Quantity: 50 reactions
Description:
NEB* Golden Gate Assembly kit, Updated to include BsaI-HFv2, Seamless cloning -no scar, plasmid with T7/SP6 promoters, Efficient with regions with high GC content and areas of repeats, Compatible w/ broad range of fragment sizes < 100 bp to > 15 kb, 100 rxns
Description:
Gibson Assembly* HiFi 1 Step Cloning Kit, Component: GA HiFi 1 Step Master Mix (2X), 50ul,GA Positive Control (2X), 10 uL (2 Control Reactions),Detection Range: 200 bp to 100 kb, up to 5 fragments in one reaction, Method: One-step, Quantity: 10 reactions
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