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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Description:
Ampligase* DNA Ligase Kit, catalyzes NAD-dependent ligation, Contains: Ampligase DNA Ligase, 10X Reaction Buffer, and Control DNA. Ampligase Enzyme & Buffer contain: Ampligase DNA Ligase and 10X Reaction Buffer (25 u
Description:
Ampligase* DNA Ligase, for applications where ligation at high temperature is beneficial, High thermostability allows ligation using high-stringency hybridization condition, High specificity and stringency permits sensi
Description:
Ampligase* 10X Reaction Buffer, High specificity and stringency permits sensitive detection of SNPs11, igase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA stru
Description:
Ampligase* DNA Ligase, for applications where ligation at high temperature is beneficial, High thermostability allows ligation using high-stringency hybridization condition, High specificity and stringency permits sensi
Description:
Components: T7Select EcoR I/Hind III Vector Arms, T7Select Control Insert, T7 Packaging Extracts, T7Select Packaging Control DNA, BLT5403, BLT5615, and BL21 glycerol stock, T7SelectUP primer, T7SelectDOWN primer, Storage +2degreeC to +8degreeC
Description:
Ampligase* Enzyme and Buffer, Ligase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures, High specificity sensi
Description:
Ampligase* Enzyme and Buffer, Ligase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures, High specificity sensiti
Description:
Ampligase* Thermostable DNA Ligase with Enzyme and Buffer, atalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are s
Description:
SplintR* Ligase, also known as PBCV-1 DNA Ligase or Chlorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand, 1, 250 units
Description:
NEBNext* Ultra* II Ligation Module, optimized to efficiently ligate DNA adaptors compatible with Illumina* sequencing to end-repaired, dA-tailed DNA fragments, Reagents Supplied: NEBNext Ultra II Ligation Master Mix, NEBNext Ligation Enhancer, Size: 24 reactions
Description:
Modifying enzyme. For ligation, mutagenesis, cDNA cloning. Concentration: 1-3 units/uL. Size: 100 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information insert. 24-month lifetime.
Description:
NEBNext* Ultra* II Ligation Module, optimized to efficiently ligate DNA adaptors compatible with Illumina* sequencing to end-repaired, dA-tailed DNA fragments, Reagents Supplied: NEBNext Ultra II Ligation Master Mix, NEBNext Ligation Enhancer, Size: 96 reactions
Description:
ElectroLIgAse* combines T4 DNA lIgAse and an optimized, ready-to-use 2X reaction buffer containing a proprietary lIgAtion enhancer and no PEG
Description:
Applications: Blue/white color screening of pGEM-Z and pGEM-Zf (+/-) Vectors in the presence of X-Gal and IPTG. Production of cloned inserts as single-stranded DNA from pGEM-Zf Vectors or M13. Cloning: recA-.
Description:
Flexi* System, Entry/Transfer, directional cloning method for protein-coding sequences, based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient to transfer protein-coding regions, Size:5 entry and 20 transfer reactions
Description:
Provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8), 100mM MgCl2, 100mM DTT, and 10mM ATP. This buffer should be stored frozen at -20[degree]C in small aliquots to preserve the ATP.
Description:
Salt-T4* DNA Ligase, Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids, Salt-T4 DNA Ligase seals nicks for these DNA substrates, Size: 100000 units
Description:
Applications: Production of ssDNA. NM522 is supplied with the pGEM Single Strand System for the production of cloned inserts as single-stranded DNA (ssDNA).
Description:
SplintR* Ligase, also known as PBCV-1 DNA Ligase or Chlorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand, 6, 250 units
Description:
Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Description:
NEBuilder* HiFi DNA Assembly Master Mix, 50 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Description:
NEBuilder* HiFi DNA Assembly Cloning Kit, 10 reactions, used to assemble either single-stranded, oligonucleotides or different sizes of DNA fragments with varied overlaps, (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Description:
Modifying enzyme. For ligation, mutagenesis, cDNA cloning. Concentration: 10-20 units/uL. Size: 500 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information insert. 24-month lifetime.
Description:
T4 RNA Ligase 1 (ssRNA Ligase), High Concentration, Source: An E. coli strain that carries the T4 RNA Ligase 1 gene, catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acceptor, (30, 000 units/ml) - 5, 000 units
Description:
NEBuilder* HiFi DNA Assembly Master Mix, 10 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Description:
NEBuilder HiFi DNA Assembly Bundle for Large Fragments - 20 reactions DNA assembly system for seamless joining of of single-stranded oligonucleotides or multiple DNA fragments, regardless of length or end compatibility