Easy access to products and protocols for research use only in the identification of 2019-nCoV based on Centers for Disease Control and Prevention (CDC) recommendations
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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Description:
Modifying enzyme. For ligation, mutagenesis, cDNA cloning. Concentration: 10-20 units/uL. Size: 500 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information insert. 24-month lifetime.
Description:
T4 RNA Ligase 1 (ssRNA Ligase), High Concentration, Source: An E. coli strain that carries the T4 RNA Ligase 1 gene, catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acceptor, (30, 000 units/ml) - 5, 000 units
Description:
NEBuilder HiFi DNA Assembly Bundle for Large Fragments - 20 reactions DNA assembly system for seamless joining of of single-stranded oligonucleotides or multiple DNA fragments, regardless of length or end compatibility
Description:
Salt-T4* DNA Ligase, Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids, Salt-T4 DNA Ligase seals nicks for these DNA substrates, Size: 20000 units
Description:
Applications: Synthesis of fusion protein in vivo using pGEMEX recombinant plasmids. Bacterial strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase.
Description:
Modifying enzyme. For ligation, labeling, cloning. Concentration: 9-12 units/uL. Size: 500 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information (PPI) insert. 24-month lifetime.
Description:
ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 100reactions
Description:
ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 10 reactions
Description:
Taq DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing the cloned Taq DNA ligase gene from Thermus aqauticus HB8, Product Includes: Taq DNA Ligase, 10X Taq DNA Ligase Buffer with NAD+, Size: 10, 000 units (250 µl)
Description:
ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 50 reactions
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