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Since a successful protein expression is dependent on an efficient proteomic workflow, the reagents are designed to address all needs including induction of protein expression, cell lysis/extraction, capture, and purification. These substances are suitable for both small-scale and large-scale studies. Compatible with automated systems, the protein expression reagents enable regulated processing without monitoring the culture or adding any inducers during cell growth. A range of more specific reagents are available to fit any procedure.
Description:
is a highly selective thiol labeling probe. TS (thiosulfate) group is negatively charged and thus probes bearing a TS group are ideal for specifically labeling thiols exposed at the exterior cell surface because charged probes are less likely to enter cells.
Description:
MTSEA biotin-X selectively labels thiols in the cell surface under mild condition. The X-spacer should facilitate better biotin-avidin or streptavidin interaction than MTSEA biotin (90064).
Description:
Biotin BCN(Biocyclo[6.1.0]nonyne), an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1, 2, 3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells with copper in cell extracts
Description:
Like TS biotin ethylenediamine (92010) TS biotin-XX ethylenediamine is a highly selective thiol labeling probe. The XX spacer should facilitate better interaction between biotin and avidin or streptavidin.
Description:
is used to covalently attach biotin to a molecule with an aldehyde or ketone group. The aminooxy group reacts with an aldehyde or ketone group to form a stable oxime linkage in an neutral aqueous condition. The reaction is fast and can be accelerated by adding aniline as a catalyst.
Description:
Biotin-12 SE is an amine-reactive biotin derivative with a long spacer group. This product can be conjugated to AA-dUTP or AA-UTP to make biotin-16-dUTP or biotin-16-UTP, two important products used in genomic studies.
Description:
free base can be directly coupled to activated carboxylic acid groups without using an additional base. The X-spacer should facilitate better biotin-avidin or streptavidin interaction than biotin cadaverine free base (90079).
Description:
Biotin-PEO4-propionate succinimidyl ester is a water soluble biotin derivative that can be readily coupled to primary or secondary amines.
Description:
The Biotin SE Protein Labeling Kit includes everything required to label proteins with biotin. Sufficient for three labelings of 1 mg protein each.
Description:
can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.
Description:
can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.
Description:
Group: Adjuvants. Enhance the immune response to immunogens. Adjuvants are nonspecific stimulators of the immune response. When mixed with an antigen, they help to deposit or sequester the injected material.
Description:
Group: Adjuvants. Enhance the immune response to immunogens. Adjuvants are nonspecific stimulators of the immune response. When mixed with an antigen, they help to deposit or sequester the injected material.
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