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Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
11 rxn. Efficiency>1x10(9th) cfu/ug. K-12 strain ideally suited as initial cloning host due to high transformation efficiency, blue-white screening capability & recA endA mutations. Single-use 50-µl volume. Include SOC Medium and Test Plasmid.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
22 reaction kit. Includes Test Plasmid and SOC Medium. Widely used host for protein expression from pET recombinants. Suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in both lon and ompT proteases.
Description:
22 rxn. Efficiency>1x10(9th) cfu/ug. K-12 strain ideally suited as initial cloning host due to high transformation efficiency, blue-white screening capability & recA endA mutations. Single-use 50-µl volume. Include SOC Medium and Test Plasmid.
Description:
NovaBlue is a general purpose strain featuring high transformation efficiency, blue/white screening, high plasmid yields, and single stranded plasmid rescue.
Description:
11 reaction kit with Test Plasmid and SOC Medium. Host for protein expression from pET recombinants. For production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in lon and ompT proteases. pLysS expresses T7 lysozyme.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
1ml. Lysogens of bacteriophage lDE3, Suitable for production of protein from target genes cloned in pET vectors. pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme. Suppress basal expression of T7 RNA polymerase.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
1ml. Host competent cells are isogenic with the lDE3 lysogens used for protein expression with pET, pETBlue*, and pTriEx* vectors, but lack a source of T7 RNA polymerase. Standard host used for initial cloning of pET, pETBlue and pTriEx vector constructs.
Description:
11 reaction kit. Includes Test Plasmid and SOC Medium. Widely used host for protein expression from pET recombinants. Suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Deficient in both lon and ompT proteases.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
22 reaction kit. Includes Test Plasmid and SOC Medium. For general cloning purposes. Has extremely high transformation efficiency, blue/white screening of recombinants by lacZ alpha-complementation, endA and recA mutations, and F factor to support M13 growth.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
0.4ml. Host competent cells that combine the key features of BL21 (& Tuner* derivative), Origami, and Rosetta strains to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm.
Description:
1ml. Host competent cells that combine the key features of BL21 (& Tuner* derivative), Origami, and Rosetta strains to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
NovaBlue is a general purpose strain featuring high transformation efficiency, blue/white screening, high plasmid yields, and single stranded plasmid rescue.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
0.4ml. Lysogens of bacteriophage lDE3, Suitable for production of protein from target genes cloned in pET vectors. pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme. Suppress basal expression of T7 RNA polymerase.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
.4ml size. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
11 reaction kit. Includes Test Plasmid and SOC Medium. For general cloning purposes. Has extremely high transformation efficiency, blue/white screening of recombinants by lacZ alpha-complementation, endA and recA mutations, and F factor to support M13 growth.
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
Description:
0.4ml. Host competent cells are isogenic with the lDE3 lysogens used for protein expression with pET, pETBlue*, and pTriEx* vectors, but lack a source of T7 RNA polymerase. Standard host used for initial cloning of pET, pETBlue and pTriEx vector constructs.
Competent Cells
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