Easy access to products and protocols for research use only in the identification of 2019-nCoV based on Centers for Disease Control and Prevention (CDC) recommendations
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Description:
Store vector at -20[degree]C. The pSP72-pSP73 Vectors can be used as standard cloning vectors and can also be used for in vitro transcription from either strand using the SP6 or T7 promoter.
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The CellTiter 96 AQueous Assay is a non-radioactive, colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays.
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Store at -70[degree]C. Quick: One tube, one pipetting step. Complete: Reagents including RNasin Ribonuclease Inhibitor are included in the Quick Master Mix. Value: One tube format means no leftover reagents.
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Recognition/cut site: G//GATCC. Size: 2500 units. The following assays assure purity and activity: activity determination assay, overdigestion assay, and ligation assay. Includes tube of acetylated BSA. Re-assayed every 3-6 months.
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Trypsin Enhancer is designed to enhance the enzymatic performance of trypsin or chymotrypsin in any digestion reaction performed with the objective of characterizing the products by mass spectrometry or liquid chromatography.
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Store at room temperature. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. A 1X solution is obtained by adding 1 part of the TAE to 9(or 39 or 49) parts distilled deionized water.
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Promega's TNT Wheat Germ Extract Systems produce proteins in vitro directly from protein-encoding DNA cloned downstream of T3, T7 or SP6 RNA polymerase promoters.
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Store at 4[degree]C. Do not freeze!. Streptavidin Alkaline Phosphatase is used for the detection of biotinylated molecules in immunoblots, such as those produced using the PinPoint; or Transcend; Systems.
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Gotaq* Long PCR Master Mix, contains the high-performance GoTaq* Hot Start Polymerase in a specially formulated mixture with a proprietary thermostable proofreading polymerase, efficient amplification of up to 40kb from lambda DNA or 30kb, Size:100 reactions
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For sequencing, mutagenesis, cDNA synthesis, cDNA cloning, primer extension. Concentration: 5-10units/uL. 1000 units. In a tamper evident, plastic sealed packet. Contains the enzyme tube, its buffer(s) and lot-specific Promega* Product Information. 24-month lifetime.
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ADCC Reporter Bioassay, Complete (Raji), Fc receptor-mediated antibody-dependent cell-mediated cytotoxicity (ADCC), important mechanism of action (MOA) by which antibodies target disease cells for elimination, used to measure ADCC utilize primary donor peripheral blood mononuclear cells
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Was expressed by baculovirus in sf9 insect cells using c-terminal his tags. ampk is a heterotrimeric protein kinase consisting of an ? catalytic subunit, and non-catalytic ? and ? subunits.
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Store at room temperature. The Wizard Genomic DNA Purification Kit provides a simple, rapid method for the isolation of genomic DNA from whole blood, culture cells, yeast, bacteria, mouse tissue and numerous other cell sources.
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PLK1 Kinase Enzyme System, Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag, member of the Polo-Like Kinase family, Deregulated expression of human PLK1 is strongly correlated with malignancies, Size: 1 mg
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HaloTEV Protease, 81kDa, fusion between the halotag protein and tev protease, a highly specific proteolytic enzyme that cleaves at a specific tev site, a specific seven-amino-acid sequence (ENLYFQ(G/S)), allowscovalent immobilization on HaloLink* Resin, Size:200ul
Description:
Store Steady-Glo; Luciferase Assay Substrate at -20[degree]C. Store Steady-Glo; Luciferase Assay Buffer below 25[degree]C. Steady-Glo; Luciferase Assay System is designed to yield reliable and robust results in high throughput screening applications.
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Proteinase K solution, ReliaPrep* Large Volume HT gDNA Isolation System, isolates genomic DNA (gDNA) from 1 to 10ml of blood in a scalable format, eliminates tedious centrifugation steps as well as the use of hazardous chemicals, size: 23ml
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Store at -20[degree]C. The Access RT-PCR System combines the sensitivity of two-enzyme systems with the convenience of single-enzyme protocols.
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PHAb Thiol Reactive Dye, pH sensor dye, low fluorescence at pH > 7 and a dramatic increase in fluorescence as the pH of the solution becomes acidic, excitation maxima (Ex) at 532nm and emission maxima (Em) at 560nm, designed specifically for antibody labeling, Size:4x 250ug
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ViaFect* Transfection Reagent, novel formulation reagent designed to efficiently introduce DNA into a wide variety of cell line, allows cells to stay healthy and metabolically active during transfection-based experiments and offers an easy-to-use protocol, size: 2 x 0.75ml
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CellTiter-Glo* 3D Cell Viability Assay, homogeneous method to determine the number of viable cells in 3d cell culture based on quantitation of the atp present, which is a marker for the presence of metabolically active cells, for use with multiwell-plate formats, Size:10ml
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Contains two dyes (a blue dye and a yellow dye) that separate during electrophoresis to show migration progress. The buffer also contains a compound that increases sample density. This means samples can be loaded directly onto gels without the need for loading dye.
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Store at 22-25[degree]C. Protect from moisture. Urea is a protein denaturant. Urea is qualified for use as the denaturing component in polyacrylamide gels. Each lot is tested and certified to be free of DNase, RNase, and protease activity.
Description:
Recognition/cut site: GAGCT//C. 10000 (1x10000) units. The following assays assure purity or activity: activity determination assay, overdigestion assay, and ligation assay. Blue/white cloning, genome qualified. With a tube of acetylated BSA. Re-assayed every 3-6 months.
Description:
Nano-Glo* Dual-Luciferase* Reporter Assay System, homogeneous reagent system that allows you to sequentially detect the activities of firefly, Features: Experience Improved Assay Performance, Notice Improved Ease-of-Use, storage: -30 deg C to -10 deg C, size: 10x10ml
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Provides high-speed purification of plasmid dna using a newly developed silica membrane based technology column. this unique design allows midiprep purification in as little as 10 minutes
Description:
GoTaq* G2 Green Master Mix, wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency, For superior convenience with hot-start PCR, Size: 100 reactions
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