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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Description:
NEB* Golden Gate Assembly kit, Updated to include BsaI-HFv2, Seamless cloning -no scar, plasmid with T7/SP6 promoters, Efficient with regions with high GC content and areas of repeats, Compatible w/ broad range of fragment sizes < 100 bp to > 15 kb, 100 rxns
Description:
Gibson Assembly* HiFi 1 Step Cloning Kit, Component: GA HiFi 1 Step Master Mix (2X), 50ul,GA Positive Control (2X), 10 uL (2 Control Reactions),Detection Range: 200 bp to 100 kb, up to 5 fragments in one reaction, Method: One-step, Quantity: 10 reactions
Description:
Gibson Assembly* Ultra Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, Two step reactions to generate DNA with positive control (2 reactions), Quantity: 10 reactions
Description:
NEB* PCR Cloning Kit, contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. 20 reactions
Description:
Gibson Assembly* HiFi 1 Step Master Mix (2X) Cloning Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, up to 5 fragments in one reaction, One-step, 10 reactions
Description:
Packaging efficiency: 1e9 pfu/ug. Preferentially packages phage with large inserts; For genomic lambda and cosmid libraries Contains: Gigapack III XL packaging extract, control lambda DNA and control host strain 4 vials
Description:
For Academic Use Only. Novel, Robust Technology For Complex, Multi-Site Mutagenesis In A Single Reaction. Contains: Dna Polymerase Blend, Template Digestion Enzyme, Competent Cells, Positive Controls. 1 Kit Contains 30 Reactions.
Description:
High efficiency blunt-end PCR cloning kit features PfuUltra * II HS DNA Polymerase, DNA topoisomerase I, and Cre recombinase technologies 20 reactions
Description:
DNA Ligase T4 Molecular Biology Grade, Store vial at -20degree C prior to opening, Application Note: One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37degree C, 500units
Description:
Ampligase* DNA Ligase Kit, catalyzes NAD-dependent ligation, Contains: Ampligase DNA Ligase, 10X Reaction Buffer, and Control DNA. Ampligase Enzyme & Buffer contain: Ampligase DNA Ligase and 10X Reaction Buffer (25 u
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