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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Catalog Number: 77461-260
Supplier: TELESIS BIO


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Catalog Number: 77461-272
Supplier: TELESIS BIO


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Catalog Number: 77461-268
Supplier: TELESIS BIO


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Catalog Number: 97065-204
Supplier: Lucigen



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Description:   Components: T7Select EcoR I/Hind III Vector Arms, T7Select Control Insert, T7 Packaging Extracts, T7Select Packaging Control DNA, BLT5403, BLT5615, and BL21 glycerol stock, T7SelectUP primer, T7SelectDOWN primer, Storage +2degreeC to +8degreeC
Catalog Number: EM70550-3
Supplier: MilliporeSigma



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Description:   Ampligase* Enzyme and Buffer, Ligase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures, High specificity sensi
Catalog Number: 76081-598
Supplier: Lucigen



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Description:   Ampligase* Enzyme and Buffer, Ligase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures, High specificity sensiti
Catalog Number: 76081-602
Supplier: Lucigen



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Description:   Components: pSTBlue-1 Blunt Vector, Blunt Vector Positive Control Insert, T4 DNA Ligase, End Conversion Mix, Nuclease-free Water, NovaBlue GigaSingles* Competent Cells, SOC Medium, Test Plasmid, ≤ -70degreeC
Catalog Number: EM71229-3
Supplier: MilliporeSigma



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Catalog Number: 97065-200
Supplier: Lucigen



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Description:   Ampligase* DNA Ligase Kit, catalyzes NAD-dependent ligation, Contains: Ampligase DNA Ligase, 10X Reaction Buffer, and Control DNA. Ampligase Enzyme & Buffer contain: Ampligase DNA Ligase and 10X Reaction Buffer (25 u
Catalog Number: 76081-604
Supplier: Lucigen



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Description:   Ampligase* DNA Ligase, for applications where ligation at high temperature is beneficial, High thermostability allows ligation using high-stringency hybridization condition, High specificity and stringency permits sensi
Catalog Number: 76081-600
Supplier: Lucigen



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Description:   Ampligase* 10X Reaction Buffer, High specificity and stringency permits sensitive detection of SNPs11, igase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA stru
Catalog Number: 76081-596
Supplier: Lucigen



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Description:   Ampligase* DNA Ligase, for applications where ligation at high temperature is beneficial, High thermostability allows ligation using high-stringency hybridization condition, High specificity and stringency permits sensi
Catalog Number: 76081-594
Supplier: Lucigen



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Description:   PT7Blue Perfectly Blunt* Cloning Kit, Components: Blunt Vector, Positive Control Insert, End Conversion Mix, T4 DNA Ligase, Nuclease-free Water, NovaBlue Singles Competent Cells, SOC Medium, Test Plasmid, Storage ≤ -70degreeC
Catalog Number: EM70189-3
Supplier: MilliporeSigma



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Description:   PT7Blue Perfectly Blunt* Cloning Kit, Components: Blunt Vector, Positive Control Insert, End Conversion Mix, T4 DNA Ligase, Nuclease-free Water, NovaBlue Singles Competent Cells, SOC Medium, Test Plasmid, Storage ≤ -70degreeC
Catalog Number: EM70189-4
Supplier: MilliporeSigma



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Description:   Ampligase* Thermostable DNA Ligase with Enzyme and Buffer, atalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are s
Catalog Number: 76081-592
Supplier: Lucigen



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