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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   5,000 units.
Catalog Number: 101228-188
Supplier: New England Biolabs (NEB)


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Description:   1,000 units.
Catalog Number: 101228-192
Supplier: New England Biolabs (NEB)


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Description:   NEBNext* Ultra* II Ligation Module, optimized to efficiently ligate DNA adaptors compatible with Illumina* sequencing to end-repaired, dA-tailed DNA fragments, Reagents Supplied: NEBNext Ultra II Ligation Master Mix, NEBNext Ligation Enhancer, Size: 24 reactions
Catalog Number: 102969-106
Supplier: New England Biolabs (NEB)


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Description:   SplintR* Ligase, also known as PBCV-1 DNA Ligase or Chlorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand, 1, 250 units
Catalog Number: 102877-586
Supplier: New England Biolabs (NEB)


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Catalog Number: PAM8221
Supplier: Promega Corporation



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Description:   Thermostable 5' App DNA/RNA Ligase
Catalog Number: 102715-904
Supplier: New England Biolabs (NEB)


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Description:   Contains C8641, Flexi* System, Entry/Transfer; A9280, Wizard* SV Gel and PCR Clean-Up System; R1901, Carboxy Flexi* Enzyme Blend; and G3780, HaloTag* Flexi* Vectors—CMV Deletion Series Sample Pack.
Catalog Number: PAG6050
Supplier: Promega Corporation



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Description:   Gibson Assembly Master Mix
Catalog Number: 102500-054
Supplier: New England Biolabs (NEB)


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Description:   SplintR* Ligase, also known as PBCV-1 DNA Ligase or Chlorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand, 6, 250 units
Catalog Number: 102877-584
Supplier: New England Biolabs (NEB)


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Catalog Number: 76538-670
Supplier: New England Biolabs (NEB)



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Description:   T4 rna ligase 2, truncated k227q - 2,000 units
Catalog Number: 101640-958
Supplier: New England Biolabs (NEB)


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Catalog Number: 76717-036
Supplier: New England Biolabs (NEB)


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Catalog Number: 76717-054
Supplier: New England Biolabs (NEB)


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Description:   Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Catalog Number: 101386-088
Supplier: New England Biolabs (NEB)


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Catalog Number: PAM8225
Supplier: Promega Corporation



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Catalog Number: PAC8820
Supplier: Promega Corporation



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