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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   NEB* Golden Gate Assembly kit, Updated to include BsaI-HFv2, Seamless cloning -no scar, plasmid with T7/SP6 promoters, Efficient with regions with high GC content and areas of repeats, Compatible w/ broad range of fragment sizes < 100 bp to > 15 kb, 100 rxns
Catalog Number: 76326-026
Supplier: New England Biolabs (NEB)



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Description:   Gibson Assembly* HiFi 1 Step Cloning Kit, Component: GA HiFi 1 Step Master Mix (2X), 50ul,GA Positive Control (2X), 10 uL (2 Control Reactions),Detection Range: 200 bp to 100 kb, up to 5 fragments in one reaction, Method: One-step, Quantity: 10 reactions
Catalog Number: 10820-610
Supplier: TELESIS BIO



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MSDS SDS    
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Catalog Number: ST200531
Supplier: Agilent Technologies



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Catalog Number: 77461-264
Supplier: TELESIS BIO


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Description:   Quick Blunting Kit - 20 rxns
Catalog Number: 101417-942
Supplier: New England Biolabs (NEB)


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Catalog Number: ST200513
Supplier: Agilent Technologies



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MSDS SDS    
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Catalog Number: 77461-266
Supplier: TELESIS BIO


New Product

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Description:   NEB* Golden Gate Assembly kit, Updated to include BsaI-HFv2, Seamless cloning -no scar, plasmid with T7/SP6 promoters, Efficient with regions with high GC content and areas of repeats, Compatible w/ broad range of fragment sizes < 100 bp to > 15 kb, 20 rxns
Catalog Number: 76326-028
Supplier: New England Biolabs (NEB)



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Description:   High efficiency UA PCR cloning, features DNA topoisomerase I and Cre recombinase technologies 20 reactions
Catalog Number: 99901-042
Supplier: Agilent Technologies



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Description:   Gibson Assembly* Ultra Master Mixes (2X) Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, Two step reactions to generate DNA, Quantity: 10 reactions
Catalog Number: 10820-792
Supplier: TELESIS BIO



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MSDS SDS    
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Description:   The kit provides a rapid and reliable method for site directed mutagenesis. 1 kit (30 rxn)
Catalog Number: 99900-044
Supplier: Agilent Technologies



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Catalog Number: ST200517
Supplier: Agilent Technologies



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MSDS SDS    
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Description:   For Academic use only. Novel, robust technology for complex, multi-site mutagenesis in a single reaction. 1 Kit (10 Rxn)
Catalog Number: 99900-024
Supplier: Agilent Technologies



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MSDS SDS    
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Catalog Number: 77461-296
Supplier: TELESIS BIO


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Catalog Number: 77461-258
Supplier: TELESIS BIO


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Description:   DNA Ligase T4 Molecular Biology Grade, Store vial at -20degree C prior to opening, Application Note: One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37degree C, 100units
Catalog Number: 10801-154
Supplier: Rockland Immunochemical



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