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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   T4 RNA Ligase 1 (ssRNA Ligase), High Concentration, Source: An E. coli strain that carries the T4 RNA Ligase 1 gene, catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acceptor, (30, 000 units/ml) - 5, 000 units
Catalog Number: 102877-596
Supplier: New England Biolabs (NEB)


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Description:   100 units
Catalog Number: 101417-962
Supplier: New England Biolabs (NEB)


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Description:   NEBuilder HiFi DNA Assembly Bundle for Large Fragments - 20 reactions DNA assembly system for seamless joining of of single-stranded oligonucleotides or multiple DNA fragments, regardless of length or end compatibility
Catalog Number: 103218-906
Supplier: New England Biolabs (NEB)


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Description:   Salt-T4* DNA Ligase, Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids, Salt-T4 DNA Ligase seals nicks for these DNA substrates, Size: 20000 units
Catalog Number: 76407-346
Supplier: New England Biolabs (NEB)



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Description:   T4 RNA Ligase 2, truncated KQ
Catalog Number: 102715-908
Supplier: New England Biolabs (NEB)


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Description:   Applications: Synthesis of fusion protein in vivo using pGEMEX recombinant plasmids. Bacterial strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase.
Catalog Number: PAP9801
Supplier: Promega Corporation



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Catalog Number: 77025-080
Supplier: New England Biolabs (NEB)


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Description:   9 Degrees North DNA Ligase - 2,500 units
Catalog Number: 200064-420
Supplier: New England Biolabs (NEB)


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Description:   NEBNext Ultra Ligation Module
Catalog Number: 102855-130
Supplier: New England Biolabs (NEB)


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Description:   Blunt/TA Ligase Master Mix
Catalog Number: 102715-938
Supplier: New England Biolabs (NEB)


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Description:   NEBuilder* HiFi DNA Assembly Master Mix, 250 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Catalog Number: 102877-806
Supplier: New England Biolabs (NEB)


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Description:   Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Catalog Number: 101386-086
Supplier: New England Biolabs (NEB)


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Description:   T4 rna ligase 2, truncated k227q - 10,000 units
Catalog Number: 101640-956
Supplier: New England Biolabs (NEB)


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Description:   200 units.
Catalog Number: 101228-194
Supplier: New England Biolabs (NEB)


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Description:   RtcB Ligase, Concentration 15 uM, Source: RtcB Ligase is expressed as a His-tagged fusion in E. Coli single stranded RNA with a 3'-phospate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl (1,2). Ligation requires both GTP and MnCl2, Size: 25 reactions
Catalog Number: 103307-256
Supplier: New England Biolabs (NEB)


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Description:   T4 DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing a recombinant clone, Product Includes: T4 DNA Ligase, 10x T4 DNA Ligase Reaction Buffer, Applications: Cloning of PCR products, Next-gen library, Size: 400, 000 units (2000 units/µl)
Catalog Number: 103753-184
Supplier: INTACT GENOMICS, INC MS



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