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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   NEBNext* Ultra* II Ligation Module, optimized to efficiently ligate DNA adaptors compatible with Illumina* sequencing to end-repaired, dA-tailed DNA fragments, Reagents Supplied: NEBNext Ultra II Ligation Master Mix, NEBNext Ligation Enhancer, Size: 96 reactions
Catalog Number: 102969-104
Supplier: New England Biolabs (NEB)


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Description:   T7 DNA Ligase
Catalog Number: 102500-174
Supplier: New England Biolabs (NEB)


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Description:   ElectroLIgAse* combines T4 DNA lIgAse and an optimized, ready-to-use 2X reaction buffer containing a proprietary lIgAtion enhancer and no PEG
Catalog Number: 102715-942
Supplier: New England Biolabs (NEB)


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Description:   Applications: Blue/white color screening of pGEM-Z and pGEM-Zf (+/-) Vectors in the presence of X-Gal and IPTG. Production of cloned inserts as single-stranded DNA from pGEM-Zf Vectors or M13. Cloning: recA-.
Catalog Number: PAP9751
Supplier: Promega Corporation



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Certificate Certificates    
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Description:   Instant Sticky-end Ligase Master Mix
Catalog Number: 102715-944
Supplier: New England Biolabs (NEB)


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Description:   Flexi* System, Entry/Transfer, directional cloning method for protein-coding sequences, based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient to transfer protein-coding regions, Size:5 entry and 20 transfer reactions
Catalog Number: PAC8640
Supplier: Promega Corporation



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Description:   T3 DNA Ligase
Catalog Number: 102500-168
Supplier: New England Biolabs (NEB)


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Description:   20,000 units.
Catalog Number: 101228-180
Supplier: New England Biolabs (NEB)


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MSDS SDS Certificate Certificates    
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Description:   100,000 units.
Catalog Number: 101228-176
Supplier: New England Biolabs (NEB)


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Certificate Certificates    
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Description:   150 reactions, Ligase
Catalog Number: 101227-654
Supplier: New England Biolabs (NEB)


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Certificate Certificates    
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Description:   2,000 units.
Catalog Number: 101228-154
Supplier: New England Biolabs (NEB)


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Description:   T7 DNA Ligase
Catalog Number: 102500-172
Supplier: New England Biolabs (NEB)


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Description:   1,000 units.
Catalog Number: 101228-190
Supplier: New England Biolabs (NEB)


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Description:   500 units
Catalog Number: 101417-960
Supplier: New England Biolabs (NEB)


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Description:   T4 RNA Ligase 2, truncated KQ
Catalog Number: 102715-910
Supplier: New England Biolabs (NEB)


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Description:   Applications: Production of ssDNA. NM522 is supplied with the pGEM Single Strand System for the production of cloned inserts as single-stranded DNA (ssDNA).
Catalog Number: PAP2301
Supplier: Promega Corporation



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